果蔬中15種農(nóng)藥殘留快速檢測膠體金免疫層析多聯(lián)卡的研制

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Development of A Multiplex Colloidal Gold Immunochromatographic Multicard for Rapid Detection of 15 Pesticide Residues in Fruits and Vegetables

NI Weihong'， YIN Danhan’，LI Gaotian1， XIAO Hailong1，LU Huajin2， SHEN Guofang' （1.Hangzhou Institute for Food and Drug Control Science，Hangzhou 31oo18， China; 2.Hangzhou Aomin Biotechnology Co.， Ltd.， Hangzhou 310019， China）

Abstract： In order to meet the needs of rapid detection of a variety of specific pesticide residues in fruits and vegetables，based oncoloidal gold immunochromatography technology，this paper developed acolloidal gold immunochromatographic multicard for the simultaneous rapid detection of 15 pesticide residues，such ascarbofuran， methocarb and profenofos，by optimizing the key factors such as coloidal gold-monoclonal antibody conjugate concentration and artificial antigen coating concentration.The test results showed that the detection limit of 15 pesticide residues was 0.01mg?kg-1 to 0.3mg?kg-1 ， no false negatives were found， the false positive rate was less than （20 5% ，and the specificity and accuracy were greater than 95% .A total of2 516 batches of samples were tested， and 6 batches of positive samples were detected.This method has good accuracy and simple operation，and is suitable for high-throughput rapid screening and detection of pesticide residues in vegetables and fruits.

Keywords： fruits and vegetables; pesticide residues; rapid detection; coloidal gold immunochromatographic multicard

我國各大農(nóng)貿(mào)市場的果蔬農(nóng)殘監(jiān)測以快速檢測為主，其中膠體金免疫層析技術(shù)（GoldImmuneChromatographicAssay，GICA）因其便捷性成為主流方法[1-5]。（剩余4429字）