高效液相色譜法同時(shí)測定食品中8種非留體抗炎藥

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Simultaneous Determination of Eight Non-Steroidal AntiInflammatory Drugs in Food by High Performance Liquid Chromatography

LUO Haiyan， HE Xi* ，YANG Fang， HAO Rui， YANG Fan， GU Jinhong （Bayinguoleng Mongolian Autonomous Prefecture Institute for Food and Drug Control， Korla 8410oo，China）

Abstract： A high-performance liquid chromatography method was established to determine the content of 8 kinds of non-steroidal anti-inflammatory drugs illegally added to food. 1g of the sample was weighed， 10mL of 1% glacial acetic acid methanol solution was added for ultrasonic extraction， separated by C18 column （250mm× 4.6mm ， 5μm ），and thephosphoric acid aqueous solution with pH=2.5 wasused as mobile phaseAand methanol as mobile phase B for gradient elution，and the detection wavelength was 220nm ，and the external standard method was used for quantitative quantification.The results showed that the 8 NSAIDs had a good linear relationship with their corresponding chromatographic peak areas in the range of 0.5μg?mL-1 to 20.0μg?mL-1 ，and the correlation coefcients were all above 0.999.The method has good acuracy and precision，and is suitable forthe detection of non-steroidal anti-inflammatorydrugsinfood.

Keywords： high-performance liquid chromatography; non-steroidal anti-inflammatory drugs; food

非甾體抗炎藥（Non-Steroidal Anti-InflammatoryDrugs，NSAIDs）是一類非類固醇激素類藥物，臨床上被廣泛用于多種免疫功能紊亂炎性疾病及各種疼痛癥狀的緩解，副作用較為明顯，主要會累及胃腸及皮膚附件系統(tǒng)[1-4]。（剩余3854字）