HPLC法測定糧食中玉米赤霉烯酮不同前處理方法對比研究

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Comparative Study on Different Pretreatment Methods for Determination of Zearalenone in Grain by HPLC

LIU Min， WANG Xiaowei， ZHU Dawei， CUI Yan， ZHU Meijie （Food and Strategic Reserves Guarantee Center of Zibo， Zibo 255039， China）

Abstract： The effcts of different pretreatment methods on the analysis of zearalenone （ZEN） in grain were explored.In this paper，the effects of three extraction methods （oscilation，vortex，ultrasonic treatment） and two purification methods （to concentrate the eluent by nitrogen blowing or not）on the analysis of ZEN by high performance liquid chromatography （HPLC） were investigated by recovery and standard substances detection， and compared with the national standard method.The results indicated that the three extraction methods and two purification methods for pretreatment of grain samples could met the detection requirements. Considering improving the work efficiency，reducing operation process and saving the cost， the optimum combination of treatment methods was obtained that the extraction method was vortexing for 5min and ultrasonic treatment for 20min ，and the purification method was diluting the eluent directly with water.

Keywords： zearalenone; grain; pretreatment; high performance liquid chromatography

玉米赤霉烯酮（Zearalenone，ZEN）是一種主要由鐮刀菌屬菌株產(chǎn)生的真菌毒素，常污染玉米、麥類等谷物，具有很強(qiáng)的雌性激素作用，還具有免疫毒性及基因毒性[]。（剩余3668字）