柱前衍生-HPLC法測(cè)定桑枝總生物堿含量

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Determination of Total Alkaloids in Mulberry Branch by Pre-column Derivatization and HPLC

YUWei QIN De-song TANG Xue-mei LI Yue-ping ZHOU Chun-hong （Chongqing Food and Drug Inspection and Testing Institute）

Abstract： Objective： To establish a pre-column derivatization and high performance liquid phase method for the determinationof totalalkaloids in mulberry plants.Methods： Samples werecolected with water and injected with 9-chloroformate methyl fluorene derivative reagent by HPLC with the volume of 20μL . The chromatographic analysis was performed at with red spectrum RDC18 5μm 4.6x250mm ） on the column and gradient elution of （citric acid buffer pH4.2 ）（A）-acetonitrile （B） B at 0～22min ， 47.5% Bat ， 37.5% B→37.5% Bat 22.01～38min 37.5% B→75% Bat 75% Bat ）. Theflow rate was 1.5 . Results： In the linear range of ， the linear relationship was good ， and the detection limit was . Conclusion： The method is rapid， simple and sensitive， and can be used for the determination of total alkaloid content in mulberry branch.

Keywords：mulberry branch，total alkaloids of mulberry plant，content determination， antifungal， pre-column derivation，RP-HPLC

桑枝是?？浦参锷＃∕rousalbaL.）的干燥嫩枝，已被收錄于2020版《中華人民共和國(guó)藥典》中的一味中藥，有祛風(fēng)濕、利關(guān)節(jié)等作用。（剩余3865字）