PbCYP86A1/B1調(diào)控梨果皮褐色形成的機制研究

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中圖分類號 S661.2 文獻標識碼A

文章編號 0517-6611（2025）11-0079-07

doi：10.3969/j.issn.0517-6611.2025.11.018

開放科學（資源服務）標識碼（OSID）：

Study on theMechanism of PbCYP86A1/B1 Regulating the Formation of PearPeel Brown Color

LIFei，JIANGPing，HNGWei（1.ChzhouAgriculturalandRuralTechnologyCnter，Chuzhou，Anui2390O;2.AnuiAgiculural

University，Hefei，Anhui ） AbstractObjetie]ThereltioshipbetweenPCYP86A1，PCY86B1genesandpearplbrowntraitswasexplored.MethodThePbCYP86A1/B1genewasclonedfromthepelof“Dangshansu”pearand“Dangshanjinsu”pearbyreverse ranscriptionpolymerasechainreaction（RT-PCR）.Thevirus-inducedgenesilencing（VIGS）methodwasusedtosilencethetargetgeneusingthetobacocrispycrackvirus （TRV）vector.Byanalyzing thecontrol group（TRV2-TRV1）andthe experimental group（TRV-PbCYP86A1，TRV-PbCYP86B1）pearpericarppheotyptomaticatiolatiexpressonoftgenedangsinompositioftesberin.Resultsuls showedthatafterTRV-PbCYP86AlandTV-PbCYP86Blwereinjectedintothepearepidemis，suberinintheinjectionpartscouldnotbe accumulatedwittfrutdvelopntadidihelowgreetcoldierebeweeneijeonsitendesurronger icarpwassignificantlygreatertantatoftecontrloup.-PCRidcatedthatthxpressosofYP86A1andPCYP86Bgeesre ducedsignfcantlywithsignifcantdierenceintheexocarof‘Xiusu’pearwithVIGScomparedihontrol.Conlusion]eresultsofsuberincontentanalysisshowdthatbothPCP86AlandPCYP86BgensilencingcouldsignificantlyihibithsynthesisofC6doy fatty acids and -diacid in pear pericarp，which will reduce the total amount of suberin.

Key WordsPear; PbCYP86A1 gene; PbCYP86B1 gene;Suberin; Virus induced gene silencing

“碭山酥梨”（Pyrusbretschneidericv.‘Dangshansuli’），屬于薔薇科白梨系統(tǒng)，黃綠色表皮，但其芽變品系“碭山金酥”呈褐色，通過分子標記確定為遺傳性變異[1]。（剩余14893字）