肉制品中大腸埃希氏菌O157:H7實(shí)時(shí)熒光PCR檢測(cè)方法的研究

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摘 要：以大腸埃希氏菌O157：H7菌體抗原基因rfbE基因?yàn)榘袠?biāo)，設(shè)計(jì)特異性引物和探針，建立大腸埃希氏菌O157：H7實(shí)時(shí)熒光PCR檢測(cè)方法。對(duì)方法的特異性與靈敏性研究，并選取市售的10種肉類制品驗(yàn)證方法可行性。實(shí)驗(yàn)結(jié)果表明該方法具有良好的特異性和靈敏性，具有很好的研究?jī)r(jià)值和應(yīng)用

前景。

關(guān)鍵詞：大腸埃希氏菌O157：H7；實(shí)時(shí)熒光PCR；檢測(cè)

Detection of Escherichia coli O157：H7 by Fluorescence Real-Time PCR Method in meats

YANG Di

（Dalian Center of Inspection and Testing Certification Technical Service， Dalian 116600， China）

Abstract： A fluorescence real-time PCR method was developed to detect the Escherichia coli O157：H7. Species-specific primers and probe were designed according to the conserved sequence of rfbE gene of Escherichia coli O157：H7. The specificity and sensitivity of the method were studied， and 10 meat products were selected to verify the feasibility of the method. The results showed this method has good specificity and sensitivity， and this method is valuable for research and application prospects.

Keywords： Escherichia coli O157：H7; fluorescence real-time PCR; detection

大腸埃希氏菌O157：H7（E. coli O157：H7）是腸出血型大腸埃希氏菌的一種主要血清型，是一種危害嚴(yán)重的食源性病原微生物，其廣泛分布于自然界，嚴(yán)重威脅公眾健康[1-3]。（剩余3376字）