烤煙K326的NtALS基因單堿基編輯與抗除草劑材料創(chuàng)制

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關鍵詞：乙酰乳酸合成酶；烤煙K326；除草劑；基因編輯

中圖分類號：S572 文獻標識碼：A

文章編號：0439-8114（2025）05-0102-03

DOI：10.14088/j.cnki.issn0439-8114.2025.05.015 開放科學（資源服務）標識碼（OSID）：口

Tobacco's NtALS gene editing to develop herbicide-resistant new elite of K326

SUN Xiao-qiong1，2，ZHANG Xiao-lian3，GUO Yu-shuang3，HUJian-lin1，HUPeng1，ZHENG Xing-fei1，，WANG Hongbo1，XUE Lian1，XU De-ze1， ZHONG YU-ping2，YIN De-suo1，WANG Feng2 （1.InstituteofFoodCrops，HubeiAcademyofAgriculturalSciences，Wuhan43064，China;2.Hubei HuichuzhiBiotechCompany，Wuhan 430064，China;3.Guizhou Academy of Tobacco Science，Guiyang 55oo81，China）

Abstract： In order to breed new varieties of flue-cured tobacco resistant to herbicide，the NtALS gene of flue-cured tobacco K326 was edited by the single base，the 194th proline triad code CCA of tobacco NtALS gene was replaced with leucine encoding CTA and TTA. Sequencing results showed that the editing rate was up to 20.69% ，the identification results of the herbicide spraying resistance of positiveedited plantsshowedthatheresistancewasobvious，andanewflue-curedtobacco materialwithherbicideresistance was developed.

Key words：acetolactate synthase（ALS）；tobacco K326；herbicide；genome editing

乙酰乳酸合成酶（AcetolactateSynthase，ALS）是存在于幾乎所有植物生長中的質(zhì)體酶，也存在于一些細菌和酵母中。（剩余4168字）