大豆花葉病毒半夏分離物外殼蛋白多克隆抗體的制備

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關(guān)鍵詞：半夏（Pinelliaternata）；大豆花葉病毒；外殼蛋白；原核表達(dá)；多克隆抗體

中圖分類號(hào)： S432.4+1 文獻(xiàn)標(biāo)識(shí)碼：A

文章編號(hào)：0439-8114（2025）05-0080-03

DOI：10.14088/j.cnki.issn0439-8114.2025.05.012

Preparation of polyclonal antibody against coat protein of pinellia isolate of soybean mosaic virus

LI Xin-yun， ZHOU Yi，F(xiàn)ANG Shou-guo （Collegeof Agriculture，Yangtze University，Jingzhou 434025，Hubei，China）

Abstract：Toestablish arapid detection methodforsoybean mosaic virus（SMV），the primary pathogencausing mosaic disease in Pi neliaternata，thecoatproteingeneofSMVwasamplifiedbyreversetranscription-polymerasechainreaction（RT-PCR），anditsro karyoticexpressionvectorwasconstructed.Thepurifiedfusion-expressd proteinwasusedasanantigen toimmuizerabbitsforthe preparation of polyclonal antibodies.The results showed that the prepared antiserum exhibited a titer of 1：102，400 as determined by enzyme-linked immunosorbent assay（ELISA），a Western blot detection limit of 300ng for the antigen，and demonstrated strong specificity.

Key words：Pinellia ternata；soybean mosaic virus；coat protein；prokaryotic expression；polyclonal antibody

半夏（Pinelliaternata）為天南星科植物，以其塊莖入藥，主要有鎮(zhèn)咳止痰、鎮(zhèn)靜安眠等作用，并且其在對(duì)抗新型冠狀病毒肺炎中發(fā)揮了一定作用[2]。（剩余4235字）