廣西積雪草內(nèi)生真菌多樣性分析

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關(guān)鍵詞：積雪草[Centellaasiatica（L.）Urban]；內(nèi)生真菌；分離鑒定；多樣性；廣西

中圖分類號：Q948.12;Q949.32 文獻(xiàn)標(biāo)識碼：A

文章編號：0439-8114（2025）05-0090-12

DOI：10.14088/j.cnki.issn0439-8114.2025.05.014 開放科學(xué)（資源服務(wù)）標(biāo)識碼（OSID）：

Analysis of endophytic fungal diversity of Centella asiatica of Guangxi

LIANG Lu ?1 ，LIANG Wen-jing2，SONG Li-sha2，WAN Ling-yun2，TANGui-yu2，ZHANG Zhan-jiang2， PAN Li-mei2， ZHANG Kun2，TU Dong-ping1，WEI Shu-gen1，2 （1.FacultyofPhrmacy，GuangxiUniversityofChineseMedicine，aning530oo，China;.GuangxiBotanicalGardenofMedicialants/ Guangxi Key Laboratory of High-QualityFormation and Utilizationof Dao-Di Herbs，Nanning 530o23，China）

Abstract：Inordertoinvestigatethedistributionofendopyticfungiintherots，stemsandleavesofCentellasiatica（L.）Urban， thecompositionofendophyticfungi groupsofCentellasiaticainGuangxiwasanalyed.HealthyCentellasiaticaspecimenswerecollectedfromsixdistinctregionsinGuangxi，ncludingLiuzou，Qinzou，Beihai，Guilin，HezhoundBaiseities，folowedbydo phyticfungalisolationfromtheirroot，stem，andleaftissues.Theendophyticfungiwereidentfiedbycombining morphologicaland molecularbiologicalmethods，followedbydiversityanalysis.Theresultsshowdthatatotalof114endophticfungalstrainsweeiso lated，belongingtoplum，3classes，9orders，2fmilies，and17genera，withtetaxonomicstatusof1geusreminigudetermined.ThegeneraColetotricumandDiaporthewereidentifidastedominantendohtcfungiinCentellasiatica.Thedopt icfungidiversityofCentellasiaticaindiferntregionswassgnificantlydiferent.TheendohicfungiinGuilinCityndLuzou City were themostabundant，andtheendopyticfungiinBeihai CityandQinzhouCitywere the mostsimilar，folowedbyGuilinCity andLiuzhouCity.Throughcomparativeanalysis，itwasfoundthatheendophyticfungiinrootshadthehighestabundance，andthe similaritycoeficientofendophticfungiinstemsandlavesasthehighest.ThefindingsindicatedthatCentellasiaticinGuangxi exhibitedhighdiversityandrelativelyunifordistributionofendophyticfungi，withbothgrowthnvironmentandplantisuetypesignificantly influencing their community composition and diversity.

KeyWords：Centella asiatica；endophytic fungi；isolation and identification；diversity；Guangxi

收稿日期：2024-10-10

積雪草［Centellaasiatica（L.）Urban]為傘形科植物，是中國傳統(tǒng)中藥材。（剩余14690字）