6種病毒復(fù)合侵染“瑪莎莉”甘薯的鑒定和分析

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中圖分類號S435.31文獻(xiàn)標(biāo)識碼A文章編號 0517-6611（2025）12-0141-04

doi：10.3969/j.issn.0517-6611.2025.12.032

開放科學(xué)（資源服務(wù)）標(biāo)識碼（OSID）：

Identification and Analysisof a Mixed Infection of Six Viruses in Sweet Potato Cultivar“Mashali"

ZHANG Xiao-yan1，LIUYu-shan’，ZHU Wen-l2etal（1.Shandong（Linyi）InstituteofModerAgriculture，ZhejiangUnivesiy，iiyi，Shandong 276OoO;2.Linyi Vocational University of Science and Technology，Linyi，Shandong 276034）

AbstractTranscriptomesequencingandRT-PCRwereperformedonfourswetpotatosamplescolectedfromLinyiCityShandongProvince，tostudyttysofusttfeedswpotato“Mashl，noertrovidfercforhdigoffotato sedlingsandthefoulationofprevetionandcotroltrategies.Theseetpotatosamplesof“ashali”showdobviousosaicndcrikling symptoms.Sixknoviuses，incdingsweepotatofatheryotlevirus（PFV），swetpotatolatenvius（SPLV），sweepoatoeafcurl virus（SPLCV），sweetpotatovirusG（SPVG），sweetpotatovirusC（SPVC）andsweetpotatovirus2（SPV2）wereidentified.Thspecific primersoftheaboveviruseswereseletedforRPCRdetectio，tetargeandsiththeexpectedsizeofthesixviuseswereobaidand thetargetviussequences wereverifdbysequencing.TeresultsofRT-CRandtranscriptomesequencingwereconsistentwittesulsf 3samplescontaining6virusesand1samplecontaining4viruses.TephylogeneticanalysisbasedontheCPgeneshowedthattheetected SPFMV hadacloseevolutionalrelationshipwithSPFMVrusetcrack（RC），thedetectedSPLCV，SPV2andSPVCwere mostcloselyrelated totheisolatesofina，PVGasostoelyelatedtoholatesofCndtheeresiantfrecsogisolatefroV colectefrodftgiouloftsdyillprvdealsisforstablstofldotroldvfr nology system of sweet potato cultivar“Marasali”.

Keywords Sweet potato virus disease;Mixed infection;Transcriptome;RT-PCR

甘薯[Ipomeabatatas（L.）Lam]是集飼料、糧食和工業(yè)原料于一體的多用途作物，是我國種植面積較大的作物之一。（剩余8280字）