不同光質(zhì)處理下糙皮側(cè)耳qRT-PCR內(nèi)參基因的優(yōu)化

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中圖分類號(hào)： S646.1+41 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1673-2871（2025）06-035-09

Optimization of reference genes for qRT-PCR of Pleurotus ostreatus under different lighting treatments

HUMengdan， ZHUMengke， ZHANG Peng， GAO Yuqian，QIULiyou，LI Yanan （CollgeofLifeiensHennAgicturalUesitKeyboratyofrictualcbalEeEngengstfA ricultureandRuralAffairs，Zhengzhou45oo46，Henan，China）

Abstract：To identify suitable reference genes for analyzing the specific gene expressonof Pleurotus ostreatus under differentlightingtreatments，themyceliumof P. ostreatuswasused asthematerial，and12 geneswere screened fromthe transcriptome data of mycelium under different lighting treatments as candidate reference genes forqRT-PCRof P. ostreatus，and their expression abundanceand expresionstabilitywereevaluated.Theresults indicatedthatthe primersof12 candidatereference genes had goodspecificity inqPCR，with single peaks in the meltingcurve，andthe CTvalues of the genes were between18and26，demonstrating good gene expressionabundance.Theexpression stabilityanalysisof geNorm，NormFinderand BestKeepershowedthatthe expressionstabilityofeukaryotic translationinitiation factorl encodinggene（eIF1），ubiquitin-conjugating enzyme encoding gene（UBC），40S ribosomal protein encoding gene（40SRP）and actinl encoding gene（Actin1）were atthe forefrontin thethree analyses.The expression abundanceof these four genes underdifferent lighting treatments from high to lowwas UBC，eIF1，Actinland 40SRP.eIF1and UBCcanbeused as the best reference gene combinationatthe high abundance expresion levelof target genes.40SRPandActinlcanbeused as the best reference gene combination at medium and low abundance expression levels of target genes.

Keywords：Pleurotus ostreatus;Reference gene;Lighting treatment; qRT-PCR

食用菌在栽培過程中需要適宜的光照1，不同品種的食用菌對(duì)光照具有不同的要求和響應(yīng)[2-]。（剩余14516字）