淹水脅迫下甜瓜幼苗的轉(zhuǎn)錄組測序及生物信息學(xué)分析

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中圖分類號：S652 文獻(xiàn)標(biāo)志碼：A 文章編號：1673-2871（2025）06-014-12

Transcriptome sequencing and bioinformatics analysis of melon seedlings under waterlogging stress

WANG Yanan， JIANG Yanfang，LIU Zefa，DANG Jiancheng， ZHANG Honglei， ZHANG Zixia （HunanUveityHumities，iencedhnologyoudiooHunina）

Abstract：Waterlogging isoneof themost widespreadand frequent natural disasters worldwide.Asacucurbit crop，melon exhibits relatively poor waterlogging tolerance.When melon cultivation areas experience prolongedrainfallor flooding，severeyieldloses orevencompletecropfailureoftenoccur.Inthisstudyawaterlogging tolerant melonhighgeneration M13-06-3，wasused as experimental material. Transcriptome sequecing and quantitative real-time PCR（qRT-PCR） were employed to analyze diferentially expressed genes（DEGs）inroot tissues.The results revealed that 9037 DEGs were screned in M13-06-3 under waterlogging stress including 4529 upregulated and 4508 downregulated genes. qRT-PCR validationconfirmed that the expression patternsof five genes，MELO3Co03917，MELO3Co20588，MELO3C008314，MELO3C025360，and MELO3C021658，were largely consistent wiht the transcriptomedata，suggesting their potentialrole inmelon waterlogging tolerance.Thesefindings provideafoundationforfurther explorationof waterlogging resistant genes in melon.

Key words：Melon;Waterlogging stress;Real-time fluorescence quantitativePCR;RNA high-throughput sequencing; Differentially expressed gene

葫蘆科瓜類作物耐澇性較差，尤其是甜瓜，甜瓜屬一年生草本葫蘆科植物，喜溫?zé)釟夂颍L環(huán)境偏愛干燥排水良好的土壤，對澇濕環(huán)境耐受能力較弱，是我國重要經(jīng)濟(jì)作物。（剩余17114字）