黃檗葉綠體基因組密碼子偏好性分析

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Analysis ofcodonusagebiasinchloroplast genomeof Phellodendron amurense

LU Mengyue ZHOU MengmengYANG JunWU ZhenyuWANG Bowen ZOU YuYANG Hongsheng （College of Biologyand Agriculture，Jiamusi University， Jiamusi 1540o7，China）

AbstractTo explore thecodonusage biasand its major influencingfactors in thechloroplast genomeof Phellodendron amurense， the coding sequences （CDS） of the chloroplast genome of P .amurensewere utilized.Fragments shorter than 30O bp，containing stop codons，or repetitive sequences were excluded，ultimately retaining 52 valid sequences.The GC content and efective number of codons （ENC）of these sequences were calculated using Codon W and CUSP software，and the influencing factors of codon preference were further discussed by correlation analysis of codon preference parameters.The findings indicated thatthe average GC content within thechloroplast genome of P .amurense was 39.34% ，with the GC content at the three positions ranked as follows： （2號 The codon preference parameter correlation analysis revealed a preference for A and U as the third base of the codon，withan average ENCvalueof 49.13，indicating a weak codon usage bias.Natural selection pressure was identifiedas theprimary factor affecting codon usage bias.Tenoptimizedcodons were identified，which showed a significant preferenceforthe Aand U types.This research lays a data foundation and provides a referencefor gene engineering and the optimization of codons in the chloroplast genome of P amurense.

Keywords Phelldendron amurense； chloroplast genome； codon usage bias； optimal codons

葉綠體基因組的遺傳方式為單親遺傳，并具備一定程度的半自主性。（剩余6075字）