18</sub> （ 4.6mm×75mm3.5μm) ）色譜柱，乙腈（A）－含 0.2% 三乙胺的 0.05mol/L 磷酸二氫鉀溶液（磷酸調(diào) pH=3 ）（B）為流動(dòng)相進(jìn)行梯度洗脫，在 210nm 檢測(cè)波長(zhǎng)下對(duì)金線(xiàn)風(fēng)生物堿提取物進(jìn)行分析測(cè)定。結(jié)果：成功構(gòu)建了金線(xiàn)風(fēng)生物堿提取物的HPLC指紋圖譜，共識(shí)別出20個(gè)共有峰，11批藥材間相似度較高；左旋箭毒堿在一定濃度范圍內(nèi)呈良好線(xiàn)性關(guān)系（ R=0.9998 )，且方法的精密度、重復(fù)性、穩(wěn)定性及加樣回收試驗(yàn)RSD均小于 3.0% ，加樣回收實(shí)驗(yàn)的平均回收率為 98.11% 。結(jié)論：該方法具備高精密度、良好重復(fù)性和穩(wěn)定性，結(jié)果準(zhǔn)確可靠，適用于金線(xiàn)風(fēng)生物堿提取物的質(zhì)量控制與評(píng)估。-龍?jiān)雌诳W(wǎng)" />

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基于特征指紋圖譜的金線(xiàn)風(fēng)生物堿提取物質(zhì)量評(píng)價(jià)

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【中圖分類(lèi)號(hào)】R284.1 【文獻(xiàn)標(biāo)志碼】A 【文章編號(hào)】1007－8517（2025）11-0055-05

DOI： 10.3969/j .issn.1007-8517.2025.11. zgmzmjyzz202511012

QualityEvaluationofAlkaloidExtracts ofCyclea Hypoglauca Basedon CharacteristicFingerprint

ZHANG Yunyun12YANG Haichuan1.2*ZHOU Qi3 LAI Bingxia3 1.Guangxi Institution of chinese Medicine Pharmaceutical Science，Nanning 53OO22，China; 2.Key Laboratory forResearchon Quality Standards of Traditional Chinese Medicinein Guangxi，Nanning 530022，China; 3.YoujiangMedicalUniversityForNationalities，Baise533OOO，China

Abstract：ObjectiveToestablishthecharacteristicfingerprintofalkaloidextractofCyclea hypoglauca，anddevelopHPLC method for determination of ⊥- curarine content. Method The chromatography was performed on Agilent ZORBAX XDB -C （4.6 18 mm×75mm 3.5μm） column，Acetonitrile（A） -0.05mol/L potassium dihydrogen phosphate solution containing 0.2% triethylamine （ pH=3 ）（B）waselutedbymobilephasegradient， λ=210 nmwasused toanalyzeand determine.ResultsHPLC fingerprint of alkaloidextractofCycleahypoglaucawasestablished，2Ocommonpeakswereidentified，and11batches werehighlysiilar.L curarine showed a good linear relationship in a certain concentration range，with R=0 .9998.The precision，repeatability，stability and RSD of sample addition recovery tests were all less than 3.0% ，and the average recovery rate of sample addition recovery was 98.11% .Conclusion Themethod hashigh precision，goodrepeatabilityand stability，and theresultsareacurateand reliable， which is suitable for the quality control and evaluation of alkaloid extract of Cyclea hypoglauca .

Key words： AlkaloidExtracts of Cyclea hypoglauca;Fingerprints；L -curarine；HPLC

金線(xiàn)風(fēng)為防己科輪環(huán)藤屬（CycleaAm.exWight）植物粉葉輪環(huán)藤的Cycleahypoglauca（Schauer）Diels的干燥根。（剩余5282字）

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