玫瑰和桂花復(fù)配提取物的體外護膚功效研究

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【中圖分類號】R284.2 【文獻標志碼】A 【文章編號】1007－8517（2025）11－0030-05

DOI： 10.3969/j .issn.1007-8517.2025.11.zgmzmjyyzz202511007

Study on in Vitro Skincare Effect of Rose and Osmanthus Flower Compound Extract

CHEN Zhixiong1XIA Jin2ZHU Tingheng2YAN Cui1HONG Ni1CAO Ping1 1.Huzhou Jahen Industrial Co.，Ltd.，Huzhou 31300o，China; 2.Wukang Sub-district Community Health Service Center of Deqing County，Deqing 3132Oo，China; 3.College of Biotechnologyand Bioengineering，Zhejiang University of Technology，Hangzhou 310ooo，China

Abstract：ObjectiveTo investigatetheskincare efectsof Damascusroseandosmanthus flowercompound extract（ROT）on whitening，anti-oxidation，andanti-agingin vitro，various methodswereemployed.MethodsTheimpactofROTonthegrowth activityof B16F10cellswasasessedusing theCCK-8method，while the DPPHscavengingabilityandABTSscavengingabilityof ROTwas evaluated throughafreeradicalscavenging test.Theefectof ROTonmelanincontentinB16F10cels wasdetermiedusing thesodiumhydroxidecrackingmetod，andtheinhibitionofelastasebyROTwasstudiedthroughanelastaseinhibitiontestAddion aly，the eyeiitationpotentialofROTwasexaminedusingthechickembroalantoic membranemethod.ResultsTeresultsindicated that ROT concentrations ranging from 0. 1% to 2% had no adverse effects on B16F1O cell growth. Furthermore，the DPPH and ABTS test demonstrated that ROT concentrations of 0.5% to 2% exhibiteda high free radical scavenging ability of up to 90% .The melanin content influence test revealed that ROT concentrations of 0.1% to 2% significantly inhibited melanin production in B16F10 cells.Moreover，the elastase inhibition experiment showed that ROT concentrations of 0.1% to 2% effectively inhibited elastaseactivitynaconcentration-dependent manner.ThechickembryoalantoicmembranemethodconfirmedthatROTdidnotcauseeyeritation.ConclusionROTdemonstrated notableabilitiesinclearingDPPHandABTS，inhibitingmelaninproductioninB16F1cells， andinhibitingelastaseactivityinvitro，allwilesowingnoeyeiitationTesefidingssuggestthatROT，asaskinareactiesubstance，holds promise for in vitro whitening，antioxidant，and anti-aging effcts，with a certain levelof safety.

Key words：ROT；in Vitro Efficacy；Cell；Security

玫瑰，薔薇科薔薇屬灌木，是一種藥食同源植物[1]。（剩余6111字）