金黃色葡萄球菌模板制備的比較研究

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[中圖分類號(hào)]Q939.1;TS 207.4[文獻(xiàn)標(biāo)志碼]A[文章編號(hào)]1005-0310（2025）03-0045-08

Abstract：The present study displayed five diferent methods of template DNA extraction from Staphylococcus aureus cultures，including Simple boiling pyrolysis，SDS cracking，SDS combined with heat cracking，commercial kits extraction method and instrument automatic extraction. By using the conventional PCR and fluorescence quantitative PCR，the effects of the DNA template prepared by these methods for PCR test are compared.The study revealed that template DNA extracted using the SDS cracking and SDS combined with heat cracking methods was undetectable in both conventional PCR and fluorescence quantitative PCR without prior dilution.Template DNA extracted by simple boiling pyrolysis at dilutions ranging from 10υ to 105 was successfully detected by conventional PCR，while template DNA extractedusing the commercial kits extraction methodand instrument automatic extraction at a 10° dilution was undetected. In fluorescence quantitative PCR， template DNA obtained via the boiling pyrolysis at dilutions from 100 to 105 was consistently detected，while DNA at a 105 dilution prepared by the other four methods mentioned above was undetected.Given its high suitability for PCR-based detection，simplicity，and minimal laboratory requirements，the boiling pyrolysis method was identified as the optimal approach for template DNA preparation in Staphylococcus aureus PCR assays.

Keywords ： staphylococcus aureus ; PCR ;fluorescence quantitative PCR ; template DNA

金黃色葡萄球菌是一種典型的革蘭氏陽性球型細(xì)菌，可以大量分布于空氣、水、人和動(dòng)物的排泄物中，富含蛋白質(zhì)的食品如蛋奶類、肉類容易受到污染。（剩余9435字）