煙草SRS基因家族鑒定與非生物脅迫誘導(dǎo)表達(dá)分析

打開文本圖片集

中圖分類號：S572 文獻(xiàn)標(biāo)識碼：A文章編號：1007-5119（2025）02-0083-10

，鄧智超1,3，吳榮榮12，劉濤1,3，（1. 266101；2.青島農(nóng)業(yè)大學(xué)，青島266109；3.中國農(nóng)業(yè)科學(xué)院研究生院，北京 ）

Identification of Tobacco SRS Gene Family and Abiotic Stress-induced Expression Analysis

YANG Yalun12, DENG Zhichao1.3, DONG Ruonan1, PAN Xiaolu1,3, WU Rongrong1,2, LIU Tao1,3, GAO Xiaoming1* (1.InstituteofTobaccoResearchofCAAS,Qingdao266101,China;2.Qngdao Agricultural UniversityQingdao266109,Cina; 3. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 10oo81, China)

Abstract: The SH-related sequence(SS)gene family encodes aclass of plant-specific zincfinger protein transcriptionfactors hat playcrucial roles inregulating plant growth,developmentandstressresponses.Toexplorethefunctionofte SRSgenefamily n tobacco,18NSRSgeneswereidentifiedfromthetobacogenomedatabaseanddesignatedasNSRS1toNtSRS18.Bioinformatics analyseswereconductedtoinvestigatetheirproteincharacteristics，subcelularlocalization,phylogeneticrelationships，gene structures,andooterentditoallyprioaesoilysdeenttisssdsel theirresponses todrought,salt,andlowtemperaturestresswereanalyzedusingqRT-CR.Resultsrevealedthat NtSRSproteins containedtheconservedDUF702domain,withmostlocalizedin the nucleus.Phylogeneticanalysis clusteredtheNtSRSfamilyinto 5 subfamilies，with membersofthesamesubfamilyexhibitinghighlyconservedgeneand protein structures.Promoteranalysis identifiednumeroushormone-responsiveelementsand stres-responsive elementswithintheregulatoryregionsof NtSRSgenes. Expresion proilingshowed thatNtSRSgnes exhbitedsgnificant tssuespeciic expresson,withosthighlyexpressd inflower budsand stems.NotablyNSRS3and NSRS16 were stronglyinducedunderdrought,salt,andlowtemperature strescoditios. OverallthisstudyprovidesacomprehensivecharacterzationofthetobaccoSRSgenefamilyofferingatheoreticalbasisforthe genetic improvement of tobacco germplasm resources.

Keywords: tobacco; SRS gene family; abiotic stress; expression pattern analysis

SRS基因家族又稱為短節(jié)間相關(guān)序列[shortinternodes(SHI）-related sequences]基因家族或者STY(STYLISH)基因家族，該家族基因編碼一種植物特有的鋅指蛋白轉(zhuǎn)錄因子I。（剩余14945字）