(2)干燥方式對(duì)煙花有機(jī)酸含量影響顯著:煙花揮發(fā)酸總量以晾干最高,其次是程序升溫干燥和曬干,冷凍干燥最低;非揮發(fā)酸總量以冷凍干燥最高,恒溫 <img src="/qkimages/yckx/yckx202502/yckx20250209-2-l.jpg" with="34px" style="vertical-align: middle;"> 干燥最低;高級(jí)脂肪酸總量以冷凍干燥最高,晾干最低。(3)應(yīng)用PLS-DA可將7種干燥方式分為5類:冷凍干燥、程序升溫干燥、恒溫干燥、曬干、晾干,并基于PLS-DA模型VIP值,篩選出不同干燥方式的7種關(guān)鍵差異有機(jī)酸(戊二酸、油酸、硬脂酸、草酸、檸檬酸、肉豆蔻酸、苯乙酸),由此建立了5類煙花干燥方式的Fisher判別模型,其訓(xùn)練集和測(cè)試集的判別正確率分別為 9 6 . 7 % 1 100 % 。綜上,晾干、曬干或程序升溫(至 <img src="/qkimages/yckx/yckx202502/yckx20250209-3-l.jpg" with="34px" style="vertical-align: middle;"> )干燥的煙花有機(jī)酸組成更適用于煙用香料,有機(jī)酸可作為煙花資源干燥方式有效判別的特征化學(xué)成分。-龍?jiān)雌诳W(wǎng)" />

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干燥方式對(duì)煙花有機(jī)酸含量的影響及判別分析

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(1.中國(guó)農(nóng)業(yè)科學(xué)院煙草研究所,青島266101;2.中國(guó)農(nóng)業(yè)科學(xué)院研究生院,北京100081;3.山東臨沂煙草有限公司,山東臨沂276000;4.山東中煙工業(yè)有限責(zé)任公司技術(shù)中心,濟(jì)南250013)

中圖分類號(hào): 文獻(xiàn)標(biāo)識(shí)碼:A

文章編號(hào):1007-5119(2025)02-0069-06

Effects of Drying Methods on Organic Acid Contents of Tobacco Flowers and Discriminant Analysis

XU Minglei1,2, HOU Xiaodong1, ZHANG Zhongfeng1, TIAN Lei3*,WEI Yulei4,HAN Xiao1, TAN Xiaolei3,DU Yongmei1* (1.Tobacco ResearchInstituteofCAAs,Qingdao266101,China;2.Graduate SchoolofChineseAcademyofAgriculturalSiences, Beijing100081,China;3.ShandongLinyiTobaccoCo.,Ltd.,Linyi2760oo,Shandong,China; 4.TechnologyCenterofChina Tobacco Shandong Industry Co.,Ltd., Jinan 25oo13,China)

Abstract: Toexplore the utilizationoftobaccoflower in tobacco flavoringapplications and establish disriminant modelfordrying methods basedon keyorganic acids,GC/MS was employed toanalyze the diferences inorganic acid content acrossseven drying methods.Partialleastsuaes-dimantaalysis(-)asedtoidntifyeydirentialoganiccis,flodbyisher discriminantanalysis toconstructadiscriminantmodel foridentifyingdrying methods.Resultsshowedas thefollows.(l)Volatile acidsintobaccoflowerstreatedwithdiferentdryingmethodswereidentifiedasbenzoicacid,phenylaceticacidand3- methylpentanoic acid (with characteristic aroma), with total content ranging from 116 to 1 8 1 μ g / g . The total content of non-volatile acids ranged from 59.7 to 7 7 . 0 m g / g ,with the sum of oxalic acid, malic acid and citric acid content accounting for approximately 9 2 . 5 % to 9 7 . 8 % of the total. The total content of higher fatty acids ranged from 22.4 to 6 5 . 2 m g / g ,with unsaturated fatty acids (oleic acid,linoleic acid,and linolenic acid) accounting for about 5 8 . 7 % to 7 6 . 6 % of the total. (2) Drying methods significantly affected organic acidcontents: airdrying achieved thehighest volatile acid content,folowed byprogrammed heating drying andsun drying, whereas freeze drying yielded the lowest.Non-volatileacidsreached peak levels during freeze drying,whereastheir minimum content were observed in constant temperature drying. The total content of higher fatty acids was the highest in freeze drying andthelowest inairdrying.(3)PLS-DAclasifiedthesevendrying metodsintofivecategories:freezedrying,programmedheating drying,constanttemperaturedrying,sundrying,andairdrying.BasedontheVIPvaluesfromthePLS-DAmodel,sevenkey diferentialoanccids,elytaricid,leiccidiccidliccidiriccid,sticcidndacid wereidentifiedascharacteristicmarkersSubsequetlyaFisherdisrimiantmodelwasestablishdforthfivecategorsoftobac flower drying methods, with classification accuracies of 9 6 . 7 % in the training set and 1 0 0 % in the test set. In summary, the organic acid profiles of tobacco flowers subjected to air drying,sun drying,or programmed heating drying (up to )exhibitgreater suitabilityfortobacoflavoringapplications.Organicacidscanserveascharacteristicchemicalcomponentsforeffectively discriminating tobacco flower resources obtained from different drying methods.

Keywords: tobacco flowers; drying method; organic acid; PLS-DA; Fisher discriminant analysis

烤煙是我國(guó)重要的經(jīng)濟(jì)作物。(剩余14840字)

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