柱前衍生HPLC法分析煙葉水溶性多糖的單糖組成

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，(1.，廈門；2.，廈門361005）

中圖分類號： 文獻標識碼：A

文章編號：1007-5119（2025）02-0052-08

Analysis of Monosaccharide Composition of Tobacco Water-soluble Polysaccharides by Pre-column Derivatization HPLC

ZHAO Yan12,HUANG Yanjun1,LIU Xiucai1, HUANG Huafa1, LIU Jiangsheng1, YE Zhonglil, ZHANG Tingguil,LIU Zechun1, XIE Wei1*,LI Yaoqun2* (1.TechnologyCenterofChinaTobaccoFujianIndustrialCo.,Ltd.,Xiamen36101,China;2.ColegeofChemistryandCheical Engineering, Xiamen University, Xiamen 3610o5, China)

Abstract:Adeterminationmethod was established forthe monosaccharide compositionandcontentof tobaco water-soluble polysacharides by1-phenyl-3-methyl-5-imdazolinone(PMP)pre-columnderivatizationhigh performance liquidchromatography. Completeacidhydrolysis method was employedtohydrolyze tobacco water-solublepolysaccharides,andthe hydrolysis products weresubjectedtoPMPderivatizationreaction.Thesignalofmonosaccharidederivativesof tobacowater-solublepolysaccharides afteracid hydrolysis was determinedbyreversephase high-performance liquidchromatography.Themethod wasusedto explorethe monosaccharidecompositionandcontentof tobaccowater-solublepolysaccharidesofdiferentregionsandtypes.Theoptimized detection conditions were as follows: 0 . 0 5 m o l / L phosphate( -NaOH,pH7.O) buffer (solvent A) and acetonitrile (solvent B) were chosen as the mobile phase of elution system at the isocratic elution condition ( 8 4 : 1 6 ) ,with Agilent Eclipse XDB-C18 column ( 2 5 0 m m×4 . 6 m m, ; theUV detection wavelength was 2 5 0 n m .Results showed that the linear relationship is good for the nine monosaccharides in the concentration range of 2 0 . 0-8 0 0 μ g / m L ，with the correlation coefficient .The limits of detection and quantification were 0.07-0.17 and 0 . 2 2 -0 . 5 6 μ g / m L ，respectively. The average recoveriesranged 8 2 . 3 % - 1 0 4 . 0 % in spiked samples.Thetobacowater-solubleposaccharideswerecomposedofmanose (Man)hamose(Rha),galactose(Gal)aboe (Ara),glucose(Glc)，glucuronicacid (GlcUA)，and galacturonicacid (GalUA)，with Glcand GalUA acounting forahigher proportion.There were significant differences inthe monosaccharidecompositionof tobaco water-soluble polysaccharides from variousregionsandtypes.Themethodisfast,simple,highlysensitive，andhasgoodrepeatability，thuscanbeused for monosaccharidecompositionanalysisof tobaccopolysaccarides.At thesametime,itprovidesabasisforfurtherresearchonthe structure of tobacco polysaccharides.

Keywords: tobacowater-solublepolysaccharides;pre-coumnderivatizatio;iquidchromatography;monosaccaridecoositon

多糖是不同類型的單糖殘基通過多種類型的糖昔鍵連接而成的天然聚合物，與卷煙感官品質息息相關。（剩余24041字）