煙草靶斑病拮抗菌Y18鑒定及發(fā)酵條件優(yōu)化
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中圖分類號(hào)：S435.72 文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1007-5119（2025）02-0035-10
1，熊有明2，2，劉峰3，母婷婷2，彭靜4，楊未2， 1，1，5*，1*
1.，；2.，；3.，；4.，；5.，）
Identification of Tobacco Target Spot Antagonistic Strain Y18 and Optimisation of Its Fermentation Conditions
XIAO Yuel, XIONG Youming2, XIAO Zhipeng2, LIU Feng3, MU Tingting2, PENG Jing4, YANG Wei2,LI Lingling1,MEI Siyi1,LIU Tianbo5*,TANG Qianjun1*
(1.CollgeofPlantProtectio,HunanAgriculturalUniversity,Changsha428,China;2.HengyangBranchofHunanoba
Company,Hengyang 41oo1,Hunan,China;3.ongzhouBranchofHunan TobaccoCompany,Yongzhou450o,Hunan,Cina; 4.HunanPlantProtectionInstitute,Changsha41l25,China;5.HunanTobaccoResearch Institute,Changsha414,China)
Abstract:Tobacoargetspotdisease,causedbyizoctoniasolaniisoneofthemostimportantfoliariseasesoftobaccoe screeningandidentificationofbocontrolstrains,along withtheestablshmentofbocontrolfermentationconditions,cantecicaly supprtthepreventionandcontrolof thedisease,andtheaplcationofbiocontrolbacteria.Inthisstudya previouslysreeed antagoniststrainY18 wasidentifiedthroughthecombiationofmorphology,physiology,biochemistryandmolecularbiology.Using theinhibitionrateofY18onthemycelialgrowthoftargettobaccoblotchasanindex,thefermentationmediumcomponentsand fermentationconditions withtheoptimalinibitoryefectofY18onthetarget bloth were screened byone-waytest andorthogonal test.The strain Y18 was identified as Bacillusvelezensis,with the inhibitionrate of 8 1 . 6 3 % on Rhizoctonia solani. It also had pronouncedinibitiononPhytophthoracapsici，F(xiàn)usariumgraminearum，Phytophthoraparasiticavar.nicotiane,Ralstona solanacearum and Pseudomonas syringaepv. tabaci,respectively,with the inhibitory rate reaching 7 9 . 0 8 % ,and the size of the inhibition circle for Ralstonia solanacearum up to 2 7 . 2 8 m m .The optimal fermentation medium, carbon source, nitrogen source and inorganicsalt wereNBmedium,sucrose,beef pasteandmagnesiumsulphate,respectively.Theoptimalmediumcomponents were sucrose, beef paste and magnesiumsulphate.The optimal fermentation conditions were p H=6 ，fermentation timeof ,inoculum amount of 5 % and fermentation temperature of . The resultscan provide a theoretical basis for the development and utilization of strain Y18 as a biocontrol agent.
Keywords: tobacco target spot; Bacillus velezensis; antagonistic effect; fermentation optimization
煙草靶斑病是由立枯絲核菌(Rhizoctoniasolani)引起的真菌病害[1]，在我國主要煙區(qū)均有發(fā)生[2-3]，給烤煙生產(chǎn)帶來嚴(yán)重經(jīng)濟(jì)損失。（剩余31322字）