抗蟲轉(zhuǎn)基因玉米對(duì)草地貪夜蛾的室內(nèi)抗性水平鑒定

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中圖分類號(hào)：S435.132 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：1006-060X（2025）03-0061-0

引用格式：，等.抗蟲轉(zhuǎn)基因玉米對(duì)草地貪夜蛾的室內(nèi)抗性水平鑒定[J].湖南農(nóng)業(yè)科學(xué)，2025（3）：61-66

DOI：10.16498/j.cnki.hnnykx.2025.003.011

Evaluation ofResistance ofInsect-Resistant Transgenic Maize to Spodoptera frugiperda inLaboratory

XIONG Mei， SONG Jun，WEI Chao，LI Hui， YANG Qiang，DAI Xiao-hang，LIU Wen-juan （InstituteofQualittandardndTestingTechnologyResearch，ichuanAcademyofAgricuturalSciences，Chengdu06

Abstract：With the transgenic maize carrying C r y I A b and thatcarrying Vip3Aa20 asdonors， Cry1Ab and Vip3Aa20 were introduced intothemaizecutivarXianda90l'byrossbreeding.Theesistanceofinsect-resistantransgenicmaizetoSodopterafrgiperdwas evaluatedbyinvitrotisseboassayandthecontentofexogenousproteiswasdeteredbyee-linkedimmunosobntaay Theresultsofbioassayandproteidetectionconfirmedeachother.Theresultsshowedthattransgenic'Xianda90l'stablyexpreed exogenousproteinsCryAbandVip3Aa2OathighlevelsinalltssuesatallstagesandhadstronglethaleffectonS.frugiperda， demonstratingapromising commercial application prospect.

Keywords：insect-resistanttrasgenicmaize;Spodopterafrugiperda;evaluationofresistanceinlaboratory;CryAb;ViAa

隨著生物育種技術(shù)的迭代升級(jí)，轉(zhuǎn)基因作物已在全球范圍內(nèi)迅速推廣并投入應(yīng)用。（剩余9917字）