2017一2023年我國豬圓環(huán)病毒3型的遺傳變異分析

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中圖分類號：S852.65 文獻(xiàn)標(biāo)志碼：A 文章編號：0366-6964（2025）06-2857-11

Genetic Variation Analysis of Porcine Circovirus Type 3 in China from 2017 to 2023

WANG Yan1，HUANG Xinyu1，WU Guiying1，WU Wanping1，LU Qizhuang1，2* （1.College of Smart Agriculture， Yulin Normal University， Yulin 537ooo， China ; 2. Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology， Yulin 537ooo，China）

Abstract：The aim of this study was to understand the molecular epidemiological characteristics and genetic variation of porcine circovirus type 3 （PCV3） in China in recent years. In this study， 168 domestic （collected from 2017 to 2023） and 9 foreign PCV3 genome sequences were selected from GenBank database，and their phylogenetic tree construction， nucleotide homology comparison，amino acid sequence comparison， epitope prediction and gene recombination were analyzed by bioinformatics software，in order to clarify the transmission and genetic evolution law of PCV3 in China. The results showed that the genotypes of PCV3 in China can be divided into two subtypes，namely，PCV3a and PCV3b ，and both of them can be further divided into different gene clusters： PCV3a-1～PCV3a-3 and PCV3b-1～PCV3b-5 ，among which PCV3a-1 and PCV3b-2 have the largest number and the highest proportion. The whole genome sequence similarity of 177 PCV3 is between 98.3% and 100% ，and there are three main point mutation regions in the amino acid sequence encoded by ORF2 gene，without base deletion and insertion. In addition，the analysis of gene recombination shows that there is only one possible recombination event between these whole genome sequences of PCV3，which indicates that the prevalent genotypes of PCV3 in China are in a relatively stable stage. This study found a genotyping method that is likely to be widely recognized in the future through strict standard definition，and based on this genotyping method，the results obtained when analyzing the genetic variation of PCV3 in China from 2017 to 2023 are reliable，which not only provides a unified framework for the future research on genetic variation of PCV3，but also provides basic data for further revealing the molecular epidemiological characteristics， genetic diversity， vaccine design and prevention and control strategies of PCV3.

Keywords： porcine circovirus type 3；genetic evolution analysis； phylogenetic tree； reorganizationanalysis? Corresponding author：LU Qizhuang，E-mail： lvqizhuangO62@163.com

豬圓環(huán)病毒（procinecircovirus，PCV），作為圓環(huán)病毒科、圓環(huán)病毒屬的一員[1]，是一種無包膜的單鏈DNA病毒，且該病毒具有極為微小的尺寸，屬于當(dāng)前已知最小的動物病毒之一。（剩余16429字）