雞白痢沙門菌感染太行雞盲腸菌群的差異及功能分析

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中圖分類號(hào)：S831.2 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：0366-6964（2025）06-2741-11

Differences and Functional Analysis of Cecal Flora in Taihang Chickens Infected with Salmonella Pullorum

l， DING 2， 1， ’， 2， 1 ZHANG Zhenhong1，ZHOU Rongyan1*，LI Lanhui1

（1. College of Animal Science and Technology ，Hebei Agricultural University， O71oo1， ; 2. Hebei Institute of Animal Science and Veterinary Medicine， O71o0o，）

Abstract： The purpose of this study was to analyze the changes of cecal flora in Taihang chickens infected with Salmonella Pullorum. The cecal contents of 19-day-old Taihang chickens in uninfected group （group C） and infected group （group B） were collected. A final library of the 16S rRNA gene V3-V4 region was constructed，and the composition，abundance，and diversity of microbial flora in the two groups were analyzed. Metastat and LEfSe analysis were used to identify the different species between groups. PICRUSt was used to predict gene function. STAMP software was used to analyze gene function differences. The results showed that the relative abundance of potential beneficial bacteria in Lachnospiraceae ， Erysipelotrichaceae and Faecalitalea_ sp- in group C were significantly higher than those in group B（ P<0，05 ）.The relativeabundance of Clostridiales_bacterium in group B was significantly higher than that in group C （ P< 0. 05）. The prediction of PICRUSt gene function showed that the two groups were significantly enriched in six major metabolic pathways （ ?P<0.05） ，and the microorganisms in group B were more involved in CMP-sialic acid synthetase，which may afect the infection of Salmonella Pullorum. This study investigated the characteristics of cecal microflora flora using a Taihang chicken infection model of Salmonella Pullorum. It was hypothesized that Salmonella Pullorum may influence the host intestinal microbial flora through Clostridiales_bacterium and CMP-sialic acid synthase，providing reference data for research on Salmonella Pullorum infection.

Keywords： Taihang chicken；Salmonella Pullorum;PICRUSt function prediction ? Corresponding author： ZHOU Rongyan，E-mail： rongyanzhou@126. com

沙門菌是人獸共患病菌之一，主要寄生于人和動(dòng)物的腸道。（剩余17110字）