9</sup> PFU/ <img src="/qkimages/jsny/jsny202506/jsny20250620-1-l.jpg" with="27px" style="vertical-align: middle;"> ，用 2% 牛血清白蛋白（BSA)封閉 <sup>3</sup>h ，血清稀釋度1:500 ，血清反應(yīng)時間 60min ，酶標(biāo)二抗稀釋至 1:12000 后作用 45min ，TMB顯色液反應(yīng) 15min 后測定吸光度（ <img src="/qkimages/jsny/jsny202506/jsny20250620-2-l.jpg" with="49px" style="vertical-align: middle;"> ）。該檢測方法特異性良好，陽性臨界值為0.372，陰性臨界值為0.310;批內(nèi)變異系數(shù)為 2.30%～7.11% ，批間變系數(shù)為 1.37%～9.25% 。-龍源期刊網(wǎng)" />

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沙門氏菌噬菌體多克隆抗體制備及其檢測方法

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中圖分類號： R378.2+2;0939.48 文獻標(biāo)識碼：A 文章編號：1000-4440（2025）06-1233-07

Abstract：Phagesare mainly composed of proteinsand nucleic acids.Whenabacteriophage enters ananimal’s body， it mayberecognizedasanantigen，therebytriggering theproductionofantibodies.Inordertofurtherstudytheproductionof antibodies intheanimalbodyafteroraladministrationof phages，purifiedbroad-spectrum lyticSalmonellphage SCBS was usedas theantigen tochallenge theNew Zealand Great WhiteRabit.The polyclonalantibodiesagainstthe phage SCBS were prepared，andthe indirectELISA method was established.The phageantibodies were detected asfolows：phages werecoated at a concentration of 1×109PFU/mL ，blocked with 2% bovine serum albumin（BSA）for 3 h，the hyperimmune serum （ 1：500 ）was incubated for 6O min，then enzyme-labeled secondary antibody（ 1：12000 ）was incubated for 45min，the absorbance （ OD450 ）was measured after the substrate solution containing TMB reacted for 15 min.The established ELISA methoddemonstratedgoodspecificity.TepositiveandnegativethresholdvalueswereO.372andO.310.Theintra-assaycoefficientofvariationranged from 2.30% to 7.11% ，andtheinter-assaycoefficient ofvariation ranged from 1.37% to 9.25%

Key words： Salmonella；phage；antibody；indirect ELISA

隨著噬菌體的廣泛使用，噬菌體治療的安全性和免疫原性也廣受關(guān)注。（剩余8874字）

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