江蘇省雞源沙門菌的分子流行病學(xué)特征分析

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中圖分類號(hào)：S852.61 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)： 1000-4440（2025）06-1188-09

Abstract：Inorder toclarifythemolecular epidemiological characteristicsof Salmonella from chickens in Jiangsu province，1 641fecalsamplesofsickchickensfromlarge-scalechickenfarms ineightcitiesofJiangsu province werecolected toisolateSalmonella，andthesensitivityofSlmonellisolatestoantibioticsasdeterminedbymicrobrothdilutionmethod.Basedon the wholegenomesequencing technology，thecarrying status ofdrug resistance genesandvirulence genesof theisolates was analyzed.TeserotypeoftheisolateswasdeterminedbyidentifyingtheantigenicstructureofSalmonella，andtheepidemiological characteristicsofSalmonellin Jiangsu province wereanalyzedbymultilocussequence typing（MLST）andphylogenetic tre.The results showed that 266 strainsof Salmonellwereisolatedfrom1641chickenmanuresamples，andtheisolationratewas 16. 21% . The resistance rates of the isolated strains to ampicillin and sulfamethoxazole were 56.39% and 55.64% ，respectively. Multi-drug resistant bacteria accounted for 53.38% . The proportion of strains carrying sul2， blaTEM-l and tetA genes was 59.40% ，56. 77% and 39.10% ，respectively.A total of 21 ST types wereidentified by MLST，of which ST11 accounted for 49. 62% . The serotypes were mainly enteritis，pullorum disease，typhoid feverand Indiana.TheSalmonella isolateswere divided into five genetic evolution branches.The genetic evolutionrelationship between the same farm isolateswasrela

tivelyclose，andthegeneticrelationshipbetweendiferentfarmisolateswasfar.Theresultsofthisstudyprovideabasisforthe precise prevention and control of Salmonella in chickens in Jiangsu province.

Key words：Salmonella isolated fromchickens；epidemiology；serotypes；multilocus sequence typing；resistance genes；virulence genes

沙門菌以其獨(dú)特的革蘭氏陰性特性在自然界中廣泛存在，是一種重要的人畜共患食源性病原菌[]。（剩余10457字）