半夏ARF轉(zhuǎn)錄因子家族的全基因組鑒定及生物信息學分析

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中圖分類號：S567.2 文獻標識碼：A 文章編號：1000-4440（2025）06-1095-12

Abstract：Pineliaternata isanimportant medicinal plant.During itsgrowth，itisextremelysensitivetolightand temperature，andpronetothephenomenonofseedlingcollapse，whichleads toareduction inyield.Inthisstudy，basedon thegenomicdataof PinelliaternatareleasedbytheChinaNationalCenterfor Bioinformation，25membersofthePtARF familywereidentifiedanddividedintofoursub-familiesthroughphylogeneticanalysis.Theresultsofproteinphysicochemical propertyanalysis showedthatallPARFs inPinellaternatawere hydrophilicandunstableproteins，and mostPtARFs werelocatedinthecellnucleus.Thepredictionoftheprotein tertiarystructureindicatedthatthe tertiarystructuresof  PtARF family members with close genetic relationships werehighlysimilar，suggestingpotential functional redundancy among some PtARF proteins. The resultsof phylogenetic analysisandcollinearityanalysisshowed that the ARF genesofPinellia ternataand ricewerecloselyrelated.In the promoter regions of the ARF family genes in Pinelliaternata，thecis-actingelementswiththelargest number were those related to biotic and abiotic stress responses，followed byplant hormone-responsive elements，

andtheelementsrelatedtoplant growthanddevelopmentresponsesweretheleast innumber.Thisresultindicated that PtARFsmay be widely involved in theresponseof Pinellia ternata to stress.Further research results showed that PtARF couldrespond tolow-lightstressandhigh-temperaturestress，andmaybeinvolvedintheregulationofseedlingcollpseand tuberdevelopmentinPinelliaternata.Thisstudyprovidesanimportanttheoreticalbasisforstre-resistantbredingandoptimization ofcultivation techniquesofPinellia ternata.

Key Words： Pinellia ternate；ARF transcription factor family；sprout tumble；bioinformatics

半夏（Pinelliaternate）是天南星科半夏屬多年生草本植物，其塊莖含有多種生物活性成分，包括生物堿、有機酸、多糖、蛋白質(zhì)和核苷等[1]。（剩余15833字）