小鼠感染豬致病性大腸桿菌模型的構(gòu)建與病理研究

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Abstract：PorcinepathogenicEscherichiacoli，isanimportantpathogenthatcausesintestinaldisease，septicaemiaand urinary tract infections.Inordertoexploretheinjury mechanisminducedbypathogenic Escherichia coli，this experiment randomly divides 50 8-to 9-week-old Babl/c female mice into 10 groups，with 5 mice in each group.Four model groups areintraperitoneallyinjected with 300μL E.coli at concentrations of 1×106 cfu/mL， 1×107 cfu/mL， 1×108cfu/mL 1×109 （204號(hào) cfu/mLrespectively，while thecontrol groupmiceare intraperitoneallinjected withanequal volumeofsterilizedPBS. After72hoursofobservation，thehistopathological changesareobservedand thecelular inflammatory factors interleukin-2（IL-2）and interleukin-6（IL-6）aredetected.Theprokaryoticexpressionvectorexpressinggreenfluorescent protein（GFP） is transformed into pathogenic E coliEC2316 by electroporation transformation method，and the strain EC2316-GFPexpressng green fluorescence is obtained.The results show that the median lethal doseof strain EC2316 is 2×107 cfu/mL.The bacterial colonization in organs increases with rising bacterial concentration，and IL2 and IL-6 increased significantly.The introductionof GFPdid not alters the growth characteristicsof strain EC2316.In summary， the pathological changes and inflammatory responses observed in mice infected with porcine pathogenic E? coliclosely resemble those documented in naturally occurring cases.A mouse model of porcine pathogenic E .coli infection is successfullyestablished，whichprovidestheoreticalandpractical foundation forfurtherresearch intothedamage mechanisms caused byE.coli.

Keywords：Porcine pathogenic Escherichia coli; GFP;LD50;mouse model

豬致病性大腸桿菌?。≒orcinepathogenicEscherichiacoli），是危害豬養(yǎng)殖業(yè)的一大挑戰(zhàn)，研究顯示，該疾病在引發(fā)豬只死亡原因中占有顯著比例，尤其是對(duì)仔豬群體構(gòu)成了嚴(yán)重威脅[1，2]。（剩余9640字）