鐮刀菌FusariumoxysporumLHS-P1-3次級代謝產(chǎn)物的分離鑒定及腫瘤細胞毒活性研究

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Isolation， identification and tumor cytotoxic activity study of secondary metabolites from Fusarium oxysporum LHS-P1-3

ShuTing，Sun Jian，WuRongxiang，Wei Caixia，TianJingyuan，WangPei，KongFandong，Wang Cong，and ZhouLiman （KeyLaboratoryofChemistryandEngineeringoforestProducts，StateEthnicAfrsCommision，GuangxiKeyLaboatoyof ChemistryandEngineringofForestProducts，GuangxiCollaborativeInovationCenterforChemistryandEngineringofForest Products， Guangxi Minzu University，Nanning 530006）

AbstractObjective To investigate the secondary metabolites of the endophytic fungus Fusarium oxysporum LHS-P1-3 isolated from ground peanut.MethodsThe fungal secondary metabolites were isolated，purified by normal-phase silica gel column chromatography and high-performance liquid chromatography，etc.The structures were identified by ESI-MS，NMR，and other methods，and the tumor cytotoxicity was evaluated using the CCK8 method.ResultsNine compounds were isolated from the endophytic fungus Fusarium oxysporum LHS-P1-3， including one new compound （stackylineE methyl ester，1）.The activityresults showed that compound 3（eiatin J1） had strong tumor cytotoxic activity against HepG2 cells， with an IC50 value of 8.35±0.66 μmol/L ， stronger than the positive control drugs doxorubicin and sunitinib，and moderate cytotoxicity against LO2 normal kidney cells. IC50 value of （35.36±2.46 ） μmol/L . ConclusionCompound 1 is a new derivative of the para-substituted benzene， and compound 3 has cytotoxic activity against a variety of tumor cells.

Key wordsFusarium oxysporum LHS-P1-3; Secondary metabolites; Tuomr cytotoxic activity

鐮刀菌屬，隸屬于子囊菌門，其首次記載可追溯至1840年[1]。（剩余13674字）