鹽田來源極端嗜鹽真菌FusariumincarnatumGXIMD00527抗污損活性成分研究

打開文本圖片集

Studies on anti-fouling compounds from saltern-derived extreme halophilic fungus Fusarium incarnatum GXIMD00527

LiangLifen，LuChunju，F(xiàn)u Chunqing，Huang Huaqing，Chen Chunxiao，Qi Mengfan，Luo Xiaowei， Gao Chenghai Liu Yonghong， and Xu Xinya （InstituteofMarineDrugs/Guangxi KeyLaboratoryofMarineDrugs，GuangxiUniversityofChineseMedicine，Nanming 530200）

Abstract Objective To study the secondary metabolites of an extreme halophilic fungus，F(xiàn)usarium incarnatum GXIMDoo527，and their marine antifouling activities against Amphibalanus amphitrite.MethodsThe secondary metabolites were isolatedby silicagel column chromatographyand purified using high-performance liquid chromatography.Their chemical structures were elucidated bythe comparisons of nuclear magnetic resonance data with literature.Theanti-fouling activities and cytotoxicities ofallcompounds against barnacle larvae atachment were determined.ResultsEight compounds were isolated and identified as four tetramicacid alkaloids： decalintetracid A（1）， decalintetracid B（2）， equisetin（3）， 5′ -epiequisetin（4），and four 14-membered resorcylic acid lactones： zearalenone（5）， a -Zearalenol（6），5'-hydroxyzearalenol（7）， 8'-hydroxyzearalenone（8）. Compounds 1＼～5and 8 exhibited potent inhibitory efectson barnacle larval attchment，and compounds 1，4and 7 showed moderate inhibitory activities on the human colon cancer celline SW480.Conclusion The secondary metabolites of the extreme halophile fungus F. incarnatum GXIMDoo527 were significantly resistant to barnacle attchment fouling. This study firstly reported on anti-fouling compounds from the extreme halophile fungi derived from the Beibu Gulf saltern， which provided a theoretical basis for the development of natural anti-fouling agents.

Key WordsMarine fungus;Fusarium incarnatum; Secondary metabolites; Anti-fouling;Extreme halophilic fungus

海洋生物污損包括微型生物污損（細(xì)菌、真菌、微藻等污損微生物附著）和大型生物污損（藤壺、草苔蟲、管蟲、大型藻類等污損生物附著），對(duì)全球生態(tài)和經(jīng)濟(jì)造成重大影響。（剩余15877字）