基于斑馬魚模型的化合物lignicol促血管生成活性研究

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LiuChangyu1，LiJiaxun1，Wang Cong2，LiXiaobin1，ZhangYun1，andLiPeihail （1Biology Institute，Qilu UniversityofTechnology （Shandong Academy of Sciences），Ji'nan250103; 2School ofChemistry andChemical Engineering，GuangxiMinzu University，Nanning

AbstractObjectiveTo preliminarily explore the pro-angiogenic activity and mechanism of action of lignicol， based onthe zebrafish vascular injury model.MethodsVatalanib （PTK787） was used to incubate zebrafish embryos （24hpf） to construct a vascular injury zebrafish model，and they were exposed to lignicol atconcentrations of 5，10， and for 24h .Ferulic acid was used as a positive control to observe and calculate the vascular growth of zebrafish intersegmental and subintestinalvein vessels.The transcription levels of genes related to vascular growth in each group of zebrafish were determined using RT-PCR technology. ResultsCompared with the PTK787 model group，both the ferulic acid positive control group and Lignicol sample groups showed an increase in the length and numberof intersegmental vessls，as wellas the lengthof the subintestinal vein in zebrafish.Compared with the model group，lignicol （5，10， and ）significantly increased themRNA expressionlevelsof related genesinthevascular endothelial growth factor pathway，includingvegf，vegfa，vegfc，vegfR2，erk2，akt，raf，pkband plcY. ConclusionThis study explores the pro-angiogenic effect and mechanism of compound lignicol through a Vascular injury zebrafish model for the first time.It was found that lignicolhas significant pro-angiogenic activityat concentrations of 5，10， and . The mechanism might raise the transcription levels of related genes in the VEGF signaling pathway，promote the proliferation and migration of endothelial cels，and thus increase the length and number of blood vessels.This study laid the foundation for the application development ofcompound lignicol.

KeywordsLignicol; Zebrafish; Proangiogenic; Mechanism

血管生成是指在原有的血管結(jié)構(gòu)基礎(chǔ)上產(chǎn)生新血管的過程，該過程會受到多種誘導因子和抑制因子交互影響[1]。（剩余11371字）