海洋真菌Trichodermasp.MDCW-16次級代謝產物的研究

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中圖分類號：R978 文獻標志碼：A

Study on secondary metabolites of marine fungus Trichoderma sp.MDCW-16

Xu Rui1， Xu Xinyan1， Xu Huil， Chen Minjun1，Yang Xuefang1，Li Peihai2， Kong Fandong1，and Wang Cong1 （1KeyLaboratoryofChemistryandEngeeringofForestProducts，StateEthnicAfairsCommission;GuangxiKeyLaboratoryof

ChemistryandEngineringofForestProducts/GuangxiColaborativeInnovationCenterforChemistryandEngineringofForestProucts，

GuangxiMinzuUniversityNanning3ooo;EngineeringResearchCenterofZbrafishodelsforHumanDiseasesandDrugreein of ShandongProvine，iologyIstitute，QiluUniversityofechnology（ShandongcademyofSiences），Jian50103）

Abstract Objective To extract the secondary metabolites produced by Trichoderma sp. MDCW-16 from Beibu Gulf and banana wiltcaused by Fusarium oxysporum and study their activities.MethodsThe isolation and purification of compounds were performed by means of column chromatography. And their structures were elucidated through the analysis ofUV，MS and NMR.The isolatedcompounds were evaluated fortheir proangiogenic activity in zebrafish.ResultsOne new pyranone compound and 9 known compounds were isolated and identified： 4-hydroxyphenylacetic acid methyl ester （2），acetoxyphenylethanol （3），4-hydroxybenzaldehyde （4），2-hydroxy3-phenylpropionate methylester （5）， 4-hydroxyphenethyl-2-（4-hydroxyphenyl）acetate （6）， penilactone D （7），

2-（4-hydroxyphenyl）-ethanol （8）， 6-（（2S，3S）-2，3-dihydroxybutan-2-yl）-3-methyl- 2H? -pyran-2-one （9），6-（（2R，3S）- 2，3-dihydroxybutan-2-yl）-3-methyl- ?2H. -pyran-2-one （10）. Compounds 5 and 8 promoted the growth of zebrafish intersegmental vessels at the concentrations of 10 and 20μmol/L ，respectively. Conclusion Compound 1 was proved to be a new pyranone compound. Compounds 5 and 8 had proangiogenic activity.

KeywordsMarine fungi; Trichoderma sp.MDCW-16; Secondary metabolites

海洋環(huán)境具有高壓、高鹽度以及低營養(yǎng)等特點，海洋真菌具有復雜的遺傳體系，這使它們能夠產生一些與陸地生物不同的活性天然產物。（剩余12271字）