土壤來源擬盾殼霉屬真菌Paraconiothyriumsp.ACC31771的次級代謝產(chǎn)物研究

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Studies on the secondary metabolites of the soil-derived peltate fungus Paraconiothyrium sp. ACC31771

Zheng Shengyan122，He Liming2，Wang Cong1，Tao Weixin2， Zhang Keyong^，and Cai Yousheng2

（1GuangxiCollborativeInnovationCenterforChemistryandEngeeingofForestProducts，GuangxiKeyLaboratoryofCeisty

andEngineringofFrestProducts，KeyabratoryofemistrynEngieeingofForestProucts，tateEticiso SchoolofChemistrydeicalEngiiguangxiinuUvesityng30o6;hlofaceuticalic， Wuhan University，Wuhan 430o71;3DayePeople'sHospital，Huangshi 435100）

Abstract Objective For searching bioactive secondary metabolites from fungal sources， the fungus Paraconiothyrium sp.ACC31771 isolated from the soil of Kanas Lake，Burjin County， Xinjiang， China， was chemically investigated. MethodsThe soil-erived fungus Paraconiothyrium sp.ACC31771 was fermented using potato dextrose broth （PDB） medium. The fermentation broth and the mycelia were separated by filtration. The mycelia were further extracted by ultrasound with 100% acetone，and then concentrated under reduced pressure to obtain crude extract. Allcompounds were isolated and purified sequentially using silica gel column chromatography， Sephadex LH20 gel column chromatography and semi-preparative liquid chromatography. ResultsSeven known compounds were found and studied.These included decaturin B（1），15-hydroxydecaturin A （2）and oxalicine B （3）， which wereoxalicine-typealkaloids，and1，8-dihydroxy-3-methy1-9，10-anthracenedione （4），1，3，8-trihydroxy-6- methyl-9，10-anthracenedione （5）， citreorosein （6）， and （+） -2'S-isorhodoptilometrin （7）， which were anthracenediones. Compounds 5 and 7 showed inhibitory activity against Staphylococcus aureus with both MIC values of 64μg/mL ：

Key WordsSoil-derived fungi; Paraconiothyrium sp.; Structural identification; Antibacterial activity

喀納斯湖位于新疆布爾津縣，地處較高緯度并具有層狀地貌的喀納斯山區(qū)，氣候極端，植被復(fù)雜，其周圍表層土壤有機(jī)質(zhì)的含量隨海拔的升高而增加。（剩余13495字）