EB病毒編碼mRNA原位雜交和免疫組織化學(xué)CD35 CD21三染法在炎性濾泡樹(shù)突細(xì)胞肉瘤中的應(yīng)用

ApplicationofEpstein-Barrvirus insitu hybridizationand immunohistochemical CD35，CD21triplestainingininflammatoryfolliculardendriticcellsarcoma

ChenGengbiao，XuAiwei，henPeijie，Yangin

DepartmentofPathology，unYat-senemorialHospital，unYat-senUnversity，Guangzhou5Ooina

Corresponding author： Yang Min， Email： yangm225@mail.sysu.edu.cn

【Abstract】 Objective To share our insights on the immunohistochemical trichrome staining technique utilized inthediagnosisof Epstein-Barr virus-positivefolliculardendriticcell sarcoma（FDCS）.MethodsIn this study，twopatientswithEBV-positiveinflammatory foliculardendriticcellsarcoma（IFDCS）involvingtheliverand spleen，aged 24 and 68 years old respectively，and with tumor diameters ranging from 30 to 55mm ，were selected for immunohistochemical staining ofCD21and CD35，in situ hybridization forEBVencoded mRNA （EBER），and triple staining for CD35，CD21，and EBER，and reviewedthe pertinent literature.ResultsUnder the microscopeof pathological section，the fusiformoroval tumorcells were arranged inschiff-likeor fasciculararrangement.The nuclei of these atypical cells showvesicular chromatin andsmall，distinct nucleolus.The resultsof immunohistochemistry and insitu hybridizationshowedthat CD21and CD35 were positive.EBER in situ hybridization also showed positive results in2cases.The tumor cels were spindle-shaped oroval with a zonal or fascicular arrangement.Theseatypicalcells exhibited vesicular chromatinand small，conspicuous nucleoli.Immunohistochemistryand in situ hybridization revealed that the tumorcels were positive for CD21 and CD35， respectively.EBER in situ hybridization was also positive in both cases.ConclusionStaining for CD21，CD35， and EBER onthe same slide allows for the simultaneous visualization of three outcomes at both the gene and protein levels，enabling a more intuitive analysis of IFDCS tumor cells.

【Key Words】Inflammatory follicular dendritic cellsarcoma; In situ hybridization； Immunohistochemistry;CD21/CD35/EBER triple stainingDOI：10.19522/j.cnki.1671-5098.2025.03.015

濾泡樹(shù)突狀細(xì)胞（folliculardendriticcell，F(xiàn)DC）是初級(jí)淋巴濾泡和次級(jí)淋巴濾泡的抗原提呈細(xì)胞[3]。（剩余3954字）