中國特有植物吳興鐵線蓮組織培養(yǎng)再生體系的建立

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中圖分類號：S567.23 文獻標(biāo)識碼：A DOI編碼：10.3969/j.issn.1006—6500.2025.06.005

Establishment of Tissue CultureandRegenerationSystemof Clematis huchouensis Tamura，an Endemic Plantin China

SHI Jianl，QIU Guoqiang'，WU Yanfen'，WANG Huifeng2，LI Jian2，ZHANG Feng3

（1.DeqngCountyEcologicalForsetryComprehensiveCenter，Huzhou，Zejiang332o，China;2.HangzhouHongsenForstyurey PlaningandDesign CompanyLimited，Hangzhou，Zhejang310o，China;3.ZhejiangForestResourcesMonitoring，Hangzou， Zhejiang 310000， China）

Abstract：Inordertorealizeindustrialproductionandaceleratetheopularizationandapplicationprocess，itisthebstwayto establish the plant tissue culture system of Clematis huchouensis.In this study，two-year old stem segments of clematis from Xing Wu with buds wereusedas explants fortissue cultureandrapid propagation.Theresults showed thatthe mixedtreatmentof 70% （20號 alcohol sterilization for 1O sand 10% sodium hypochlorite sterilization for1O min was the best，with a germination rate of 70.67% and a pollution rate of 17.33% .1/2MS medium is more suitable for start-up culture，and the sprouts grow well，not only robust，but also without yellowing and browning.In the multiplication culture， MS+2mg?L-16-BA+0.3mg?L-1 IBA +2 ， L-1 hydrolyzed casein was the best，and the multiplication coeffcient was 4.73.The best roting culture medium was 1/2 ms+0.5 mg·L -1 IBA+0.01 mg·L-1 NAA，and the rooting rate was 79.67% .This study established a complete tissue culture technology system for Clematis huchouensis， breaking itsgegapicalitatios.Itprovidesathoreticalbasisforitspromotionandommecialdvelopent，ndlsoerse tain support for its interdisciplinary cooperation in the later stage.

KeyWords：Clematis huchouensis Tamura; sterilization; tissue culture;culture medium

鐵線蓮屬（ClematisL.）屬于毛茛科（Ranunculaceae）植物，具有較高的觀賞和藥用價值-2。（剩余6895字）