秀麗四照花組培快繁體系建立

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中圖分類號：S688.9Q945.52 文獻標識碼：A DOI編碼：10.3969/j.issn.1006—6500.2025.06.002

Establishment of Rapid Multiplication System for Tissue Culture of Cornus hongkongensis subsp.elegans RUAN Shuming，LAI Shigan， ZHOU Xianzhen

（FujianForestryVocational&TechnicalCollege，Nanping，F(xiàn)ujian353OOO，China）

Abstract：InordertoestablisharapidpropagationsystemforCornusflorida，tisseculturemethodswereused.Thestudyinvolved screenigsterilizationmethdsforstemsegmentexplantsofCorusflordandinvestigatingtheefectsofdiferentconcentratinsof hormonesNAAandBAoallsiuction，uddfrentiatio，oungleafproliferationdotigTeresultsidiatedtste ilization with 0.1% HgCl2 for 12 minuteswas optimal.Callus induction was most effectivewith M IS+1.0mg?L-16-BA+0.2mg?L-1 NAA， reaching a 90% induction rate after 40 days.Bud differentiation from callus was best promoted by MS+2.O mg·L -1 6-BA+0.2 mg·L-1 NAA. Shoot proliferation from sterile young leaves was highest with MS+1.O mg·L-1 NAA+0.5 mg·L-16-BA.Root induction was best in MS+0.5 mg·L-1 IBA，however，the induced roots were prone to browning，necessitating timely subculture onto a rejuvenationmediumIncondusion，thisstudyprovidesatechnicalfoundtionforthelarge-scalepropagatinofthisspecies，whichisof great significance for its resource development and industrial application.

Keywords：Cornus hongkongensis subsp.elegans; tissue culture; stem segment; callus

山茱萸科（Cornaceae）四照花屬（Dendrobenthamia）植物，其頭狀花序近似球形，擁有4片大型白色花瓣狀苞片，成熟時果實肉質(zhì)呈紅色，使其成為備受推崇的觀花觀果樹種，是具有較好經(jīng)濟效益的鄉(xiāng)土樹種，同時在森林生態(tài)系統(tǒng)中也發(fā)揮著重要的生態(tài)效益[2-3]，在園林綠化、果用和藥用等方面都有良好的發(fā)展前景4。（剩余9354字）