冬櫻花組培快繁技術(shù)研究

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中圖分類號(hào)：S685.99 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1002-2910（2025）03-0013-05

Research on tissue culture and rapid propagation technology of Cerasus cerasoides

XULi1，ZHANGBing2，TANYuel，ZONGXiaojuan1， ZENG Peiyuan1，WEI Hairongl （1.ShandongInstituteofomologyai'an，handong2oo，China;.iyueistrictForestryProtectiod DevelopmentCenter，Tai’anCity，Tai'an，Shandong27ooo，China）

Abstract：A rapid in vitro propagation system was successfully established for Cerasus cerasoides（D.Don）Sok.using semi-lignified stem segments as explants.The optimal surface sterilization method involved sequential treatment with 75% ethanol for 30s and 5% sodium hypochlorite solution for 10min .Theinitial culturemediumwasMS basal medium supplemented with 1.0mg/L 6-benzylaminopurine（6-BA）and 0.1mg/L indole -3- butyricacid（IBA）.The optimal proliferation medium was MS containing 0.3mg/L 6-BAand 0.2mg/L IBA，yielding a multiplication coefficient of 4.6.Rooting was optimally induced in half-strength MS medium supplementedwith 0.5mg/L α-naphthalene acetic acid（NAA），resulting in 84% rooting rate with an average of 6.24 roots per plantlet.The survival rate of transplanting using perlite + peat soil + vermiculite （volume ratio 1：3：2 ）substrateis 92%

Key words：Cerasus cerasoides;proliferation culture;rooting cultivation; transplant

冬櫻花[Cerasuscerasoides（D.Don）Sok.]為薔薇科（Rosaceae）櫻屬（Cerasus）落葉喬木，又名冬海棠，小陽春，因冬季開花而得名，是中國野生櫻花資源中唯一在冬季開花的櫻花種類[1]。（剩余5894字）