層積處理天山郁金香種子,系統(tǒng)分析了種子休眠解除過(guò)程中生理生化的變化。研究結(jié)果表明,在貯藏物質(zhì)含量方面,隨著層積時(shí)間的延長(zhǎng),種子可溶性糖含量呈先降低后升高的變化趨勢(shì),淀粉含量呈現(xiàn)先升高后降低的趨勢(shì),可溶性蛋白含量持續(xù)下降。在酶活性方面,隨著層積時(shí)間的延長(zhǎng),種子過(guò)氧化物酶(POD)活性呈先降低后升高的變化趨勢(shì)。在層積前期(1\~10 d),天山郁金香種子葡萄糖-6-磷酸脫氫酶(G-6-PDH) +6 磷酸葡萄糖酸脫氫酶(6-PGDH)的活性無(wú)明顯變化;在層積后期(40d),種子葡萄糖-6-磷酸脫氫酶(G-6-PDH) +6. 磷酸葡萄糖酸脫氫酶(6-PGDH)的活性顯著升高。內(nèi)源激素含量方面,隨著層積時(shí)間的延長(zhǎng),種子脫落酸(ABA)含量呈先升高后降低的變化趨勢(shì),赤霉素( GA<sub>3</sub> )含量呈先降低后升高的變化趨勢(shì),生長(zhǎng)素(IAA)和細(xì)胞分裂素(CTK)含量持續(xù)上升。本研究結(jié)果揭示了天山郁金香種子解除休眠過(guò)程中貯藏物質(zhì)含量、酶活性以及激素含量的變化規(guī)律,為天山郁金香種子的休眠調(diào)控和高效繁育提供了理論依據(jù)。-龍?jiān)雌诳W(wǎng)" />

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天山郁金香種子解除休眠過(guò)程中生理生化的變化

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中圖分類號(hào):S682.263 文獻(xiàn)標(biāo)識(shí)碼:A 文章編號(hào):1000-4440(2025)05-0977-07

Abstract:In this study,seeds of Tulipa thianschanica Regel were subjected to stratification at 4°C to systematically analyzethe physiological and biochemical changes during theprocess ofseeddormancyrelease.Theresults showed that in termsofseed storagesubstances,withtheextensionof stratificationtime,thecontentof solublesugarsintheseeds firstdecreasedand thenincreased,thecontentof starch firstincreasedandthendecreased,andthecontentof soluble proteinscontinuouslydeclined.Intermsofseedenzymeactivity,withtheextensionofstratificationtime,theactivityofperoxidase ( P(DD )intheseeds first decreasedand then increased.Intheearly stratificationstage(1-1Odays),theactivities of glucose-6-phosphate dehydrogenase (G-6-PDH) and 6-phosphogluconate dehydrogenase ( 6-PGDH ) in the seeds showed no significantchange.However,inthelatestratificationstage(4Odays),theactivitiesofbothenzymesintheseedssignificantly

increased.In terms of endogenous hormone content,with theextensionofstratificationtime,thecontentofabscisic acid(ABA)in theseedsfirst increased and then decreased,thecontentofgibberellin( GA3 )first decreased andthen increased,while thecontentsof auxin(IAA)and cytokinin(CTK)continuously rose.The results ofthis studyelucidated the changes in the content of storage sub

stances,enzyme activities,and hormone levels duringthe dormancyrelease of Tulipa thianschanica Regel seeds,providing a theoretical basis for the dormancy regulationand eficient propagationof Tulipa thianschanica Regel seeds.

Keywords:Tulipa thianschanica Regel;seed;dormancy;enzyme activity;hormone

天山郁金香(Tulipa thianschanica Regel)為百合科(Liliaceae)郁金香屬(Tulipa)多年生野生花卉,主要分布于中國(guó)新疆和中亞地區(qū)[1],其花色艷麗、花型獨(dú)特、抗逆性強(qiáng)。(剩余10316字)

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