馬鈴薯無糖組織培養(yǎng)的條件優(yōu)化及其在馬鈴薯原原種生產(chǎn)中的應(yīng)用

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Abstract：Tofurtheroptimizethemicroenvironmentparametersofsugar-free tisseculture，this studyused Qingshu No.9 as the tst material and designed different gradient experiments with diferentratiosof vermiculite to nutrient solution （ Vvermiculite ： 1 Vnutrientsolution=1.0：0.7，Vvemiculie：Vnutrientsolution=1.0：0.8 mtriensoution =1.0：0.9），COconcentrtions（500 μmol/mol， 800μmol/mol ， 1100μmol/mol ， 1 400μmol/mol ），and light intensities to investigate the effectsof each treatment

onthe growth and development of potato tissue-cultured seedlings.Thelight intensitywasset at 50μmol/（m2?s） 75μmol/（m2?s） ， 100μmol/（μ2?s）. The results showed that the optimal conditions for sugar-free tissue culture of potatoes were as follows ： ， CO2 concentration of 800μmol/mol ，and light intensityof75 μmol/（m2?s）. Compared with traditional tissue culture，the yield，quantity，and survival rate of potato pre-basic seeds obtained through sugar-free tissue culture were significantly increased by 16.09% ， 22.37% ，and 3.27% ，respectively（ P<

0.O5）.Inconclusion，thisstudynotonlyclarifidthekeytechnicalparametersofsugar-freetissueculturebutalsoonfimedthe advantagesoftistechologyinimprovingteproductionefiencyofpotatoseedtubers，providingteoreticalbasisadtecal support for the standardized production and industrial propagationof potato tissue-cultured seedlings.

Key words：sugar-free tissue culture；potato；tissue-cultured seedlings

植物無糖組織培養(yǎng)技術(shù)由日本千葉大學(xué)古在豐樹教授發(fā)明[1]，其技術(shù)核心在于通過精確控制微環(huán)境因素（如 CO2 濃度、溫度、濕度等），以環(huán)境 CO2 替代培養(yǎng)基中添加的外源糖作為碳源，實(shí)現(xiàn)植物完全自養(yǎng)生長(zhǎng)。（剩余10515字）