基于轉(zhuǎn)錄組解析玉米苗期根系對非生物脅迫的響應(yīng)

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中圖分類號:T513 文獻(xiàn)標(biāo)識碼:A 文章編號:1000-4440(2025)05-0848-10
Abstract:Abioticstreses(drought,salt)havebecomeoneof thekeyfactorsrestrictingtheyieldandqualityof maize. To elucidatethemechanismsof maizeresponsedtoabioticstresses,inthisstudy,B73wasusedastheexperimentalmaterial, and four treatments were set up: control (CK),drought stress treatment (D),salt stresstreatment (S)and drought + salt compoundstresstreatment(DS).Primaryroot length wasdynamicallymonitored,andtheroot transcriptome wassequenced.Theresultsofphenotypicanalysisshowedthattheprimaryrotlengthwassignificantlyshorterunderdiferenttreatmentscomparedwith the control. A total of 1 526 differentially expressed genes were identified in the comparison groups between the control and diferentstresstreatments.Atotalof2O7,17Oand1274diferentiallexpresedgeneswereidentifedinthecomparisongroupsbetween the control and drought stress,salt stress and drought + salt compound stress. Functional enrichment results suggested that thesediferentiallyexpressed genesweremainlyinvolvedinthebiologicalproceses mediatedbyabscisicacidsynthesisandsignal transduction,and transcription factors in response to abiotic stress.MYB and ERF family transcription factor genes showed the
greatest response to abiotic stress.The results of this study layafoundation for further revealingthemolecular mechanism of maize seedlings in response to abiotic stress.
Key words:maize;abiotic stress;transcriptomics;differentially expressed genes;abscisic acid
玉米(ZeamaysL.)是世界上重要的糧食、飼料和經(jīng)濟作物,在農(nóng)業(yè)生產(chǎn)和經(jīng)濟發(fā)展中占據(jù)重要地位[]。(剩余15708字)