棉花根際高抗鹽堿芽孢桿菌的篩選鑒定與功能研究

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Abstract：Soilsalinizationisoneofthemajorabioticstressesaffectingglobalagriculturalproduction.InChina，widespread saline-alkalisoilsseverelyrestrictcropyieldandqualityCottn，asanimportanteconomicropinChina，holdsasigniicant positioninthetextileindustryanditsoilandproteinarealsovitalfoodandindustrialrawmaterials.However，creased osmotic pressre，nutrientdeficiencyandsoilstructuredisruptioncausedbysoilsalinizationseverelylimitcotongrowth anddevelopment.Toaddresthischalenge，this studyaims toimprovethephysicochemical propertiesofsaline-alkali soils andthereby enhance theresistanceand yieldofcoton in saline-alkali lands through screening andutilizing Bacilus strains with high salt-alkali resistance fromcotton hizosphere.Our researchteamisolates strains with saline-alkali resistance from cottonhizospheresoilandselectstwmultifunctional，ighlysaline-alkali-resistantBacillusstrainsA3-3ndN-7though progressive salinity-alkalinity acclimation.Strain A3-3 can grow under high saline-alkali conditions of 15% NaCl and pH 9.0，and possessesarious bioactivities，includingiorganicphosphorussolubilzation，rganicphosphorusdecomposition， potassium solubilization，protease production，cellase production，andantagonismagainstcoton wilt disease，preliiarily identified as Bacillus halotolerans.Strain N-7 can growunder high saline-alkali conditions of 15% NaCl and pH11.0， and exhibitssimilarfunctionsasstrainA3-3preliminarilyidentifiedasBacilushaynesi.Potexperimentsshowthatbothstrains significantlypromotecottongrowthundermoderate toseveresaline-alkali stres.Therefore，StrainsA3-3andN-7have great potential inthe developmentandapplicationof coton-specificsaline-alkali-resistant microbial agents，providing new strategies for bioremediation of saline-alkali soils and sustainable cotton production.

Keywords：Cotton; moderate to severe saline-alkali land; saline-alkali-resistant Bacillus; function stud

土壤鹽堿化是全球農業(yè)發(fā)展中面臨的關鍵環(huán)境挑戰(zhàn)，根據土壤中所含鹽濃度及pH，將鹽堿脅迫分為三個等級[1：輕度（鹽分含量 ?0.3% ，pH7.1＼～8.5）、中度（鹽分含量 0.3%～0.6% ， pH8.5～ 9.5）和重度（鹽分含量 ?0.6%，pH9.5） ，目前對于中重度鹽堿化土壤的治理難度較大[2]。（剩余17096字）