FMNLI對膠質(zhì)瘤細(xì)胞增殖、遷移和侵襲的影響
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【中圖分類號】 R739.41 【文獻(xiàn)標(biāo)志碼】A 【文章編號】1672-7770（2025）02-0181-08
Abstract： Objective To investigate the effect FMNLl on the proliferation， migration and invasion glioma cells， and to provide a new potential target for the clinical treatment glioma patients. MethodsThe Cancer Genome Atlas（TCGA） and Chinese Glioma Genome Atlas （CGGA） databases were used to explore the relationship between FMNLl and glioma grade and prognosis patients. The effect FMNLl on the proliferation， migration and invasion glioma cells was investigated by CCK-8， cell scratch and Transwell migration and invasion function experiments. Western blot（WB） was used to detect the epithelial mesenchymal transition（EMT）in U251-shFMNLl， U251-OEFMNLl， U87-OEFMNLl and LN229-OEFMNLl cell lines Transition （EMT）， and to explore the effect FMNLl on EMT. Results The database clinical samples showed that the expression level FMNLl was positively correlated with the WHO grade glioma and significantly affected the survival time glioma patients. Knocking down or up-regulating the expression FMNLl had a significant effect on the proliferation， migration， and invasive ability glioma cells. The results WB showed that knocking down the expression FMNLl significantly elevated the expression E-cadherin expresson was significantly elevated， while the expression N-cadherin and Vimentin was significantly decreased after knocking down the expression FMNLI. After up-regulating the expression FMNLl， the expression E-cadherin was significantly decreased， while the expression N-cadherin and Vimentin was significantly increased. The expression level FMNLl is closely related to the WHO grade and the prognosis
glioma patients. As a cancer promoting factor， FMNLl can significantly improve the proliferation， migration and invasion ability glioma cell， and at the same time FMNLl promotes the EMT process glioma cells.
Key words： glioma； FMNLl ； proliferation； migration； invasion； EMT
膠質(zhì)瘤是顱內(nèi)最常見的原發(fā)性惡性腦腫瘤，大約占顱內(nèi)腫瘤的 。（剩余11970字）