MTHFD2對腦膠質(zhì)母細胞瘤細胞生長和轉(zhuǎn)移的作用和機制研究

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【中圖分類號】R739.41【文獻標志碼】A【文章編號】1672-7770（2025）02-0166-08

Abstract： Objective To explore the effects methylenetetrahydrolate dehydrogenase 2 （MTHFD2） on the growth and metastasis glioblastoma（GBM） cells and its mechanism. Methods The expression level MTHFD2 in GBM tissues and normal tissues was analyzed by interactive gene expression prile analysis database（GEPIA）. U87 MG cells were divided into control group， si-NC group and si-MTHFD2 group. The changes glycolysis and oxidative stress， as well as cell proliferation， apoptosis， migration and invasion were detected in each group. Xenotransplantation model nude mice was established and divided into sh-NC group and sh-MTHFD2 group. Tumor volume was measured and tumor growth curve was drawn. The expression levels MTHFD2 gene were detected by RT-qPCR， and the expression levels glycolysis key enzymes and epithelialmesenchymal transition marker proteins were detected by Western blot. Results GEPIA database analysis showed that MTHFD2 was highly expressed in GBM tissues （ ）. Compared with normal NHA cells， the relative expression levels MTHFD2 mRNA and protein in U87 MG cells were increased （ ）. Silence MTHFD2 inhibited the relative glucose intake and lactate production U87 MG cells， inhibited clone formation， cell proliferation activity， cell migration and invasion， and promoted the level oxidative stress and cell apoptosis.In the tumor formation experiment nude mice， silence MTHFD2 inhibited tumor growth. Conclusions MTHFD2 is a promoter malignant progression GBM. The silencing MTHFD2 expression can effctively inhibit cellular glycolysis stress， suppress cell proliferation， migration， invasion， and tumorigenesis in vivo， while also enhancing oxidative stress and apoptosis.

Key words： glioblastoma； methylenetetrahydrolate dehydrogenase 2； glycolysis； oxidativestress； proliferation and apoptosis； migration and invasion

膠質(zhì)母細胞瘤（glioblastoma，GBM）起源于星形膠質(zhì)細胞，是最具侵襲性和最常見的惡性原發(fā)性腦腫瘤，根據(jù)世界衛(wèi)生組織分類，屬于高級別惡性膠質(zhì)瘤（IV級）[1]。（剩余11410字）