基于網(wǎng)絡(luò)藥理學(xué)和分子對接技術(shù)探討菟絲子改善卵巢儲備功能減退作用機(jī)制

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[中圖分類號］R285 [文獻(xiàn)標(biāo)志碼］A [文章編號]0256-7415（2025）13-0211-10

DOl：10.13457/j.cnki.jncm.2025.13.036

Exploring Mechanism of Cuscutae Semen in Improving Diminished Ovarian Reserve Based on Network Pharmacology and Molecular Docking Technology

ZHU Yue1，LIU Ziqin1，JIN Xuening1，CUI Shu'na1.2 1.MedicalColegeInstituteofTranslationalMedicine，YangzhouUniversity，YangzhouJiangsu225o09，China；2.Departmentof Obstetrics and Gynecology，Affiliated Hospital of Yangzhou University，Yangzhou Jiangsu 225oo9，China

Abstract：Objective：To analyze the mechanism of Cuscutae Semen in improving diminished ovarian reserve（DOR）using network pharmacology and molecular docking technology.Methods：The active components of Cuscutae Semen were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（TCMSP）.Potential targets of the activecomponentsand DOR-related targetswere predicted through GeneCards，OMIM，and DisGeNET databases.A protein-protein interaction network was constructed using the String databaseand visualized with Cytoscape software.GO and KEGG enrichment analyses of the intersecting targets were performed using the Hiplot database.Finally，moleculardocking verification was conducted using AutoDock software. Results：Cuscutae Semen contains 14 activecomponents including kaempferol，quercetin，and isorhamnetin，with 58 potential targets for DOR regulation，including 10 key targets：AKT1，EGFR，TNF，IL-6，TGFB1，CASP3， MMP9，BCL2，ESR1，and TP53.The core pathways mainly included lipid and atherosclerosis，PI3K-Akt signaling pathways，etc.Molecular docking results showed good binding activity between core componentsand key targets. Conclusion：The main active components of Cuscutae Semen （kaempferol，quercetin，isorhamnetin，etc.） exert reproductive protective effcts byactingon targetssuch as AKT1，EGFR，TNF，and IL-6 to activate or inhibit signaling pathways including lipid and atherosclerosis，PI3K-Akt，etc.

Keywords：Diminished ovarian reserve；Cuscutae Semen；Network pharmacology； Target prediction；Mechanism

卵巢儲備功能減退（DOR）是指卵巢內(nèi)卵母細(xì)胞數(shù)量和（或）質(zhì)量下降，伴隨著竇卵泡數(shù)（AFC）下降，促卵泡刺激素（FSH）升高，抗繆勒氏管激素（AMH）下降。（剩余10022字）