雞白痢抗體檢測(cè)方式的改良

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中圖分類號(hào)：S852.61 文獻(xiàn)標(biāo)識(shí)碼：A 文章編碼：1005-8567（2025）03-0105-06

Abstract：To investigate the impactof cryopreservation conditionson the detection of antibodiesagainst Salmonella pullorum and the feasibility of using residual plasma after avian leukosis detection for pulorum disease detection，this study selected386O three-yellowchickens for pullorum disease testing inbatches.Thedetection results using diferent anticoagulants，cryopreserved blood samples，serum，and residual plasma were compared with the standard on-site whole blood plateagglutination detection.The results showed that the positive detection rates of

lithiumheparinanticoagulated blood，ethylenediaminetetraaceticacid dipotassumsalt（EDTAdipotassumsalt， EDTA-2K）anticoagulated bloodand residual plasma were highlyconsistent with theon-site whole blood plate agglutinationdetectionresults.The positive individuals detectedbytheon-sitewhole blood plateagglutination method could becompletelydetected inboth cryopreservedandnon-cryopreservedserumand residual plasma samples.The positive detectionrateofserumsamples was slightlyhigher thanthestandard method，andshort-termcryopreservation had no significantadverse effctonthedetectionresults.Inaddition，theactualapplication efectof using residual plasma for pullorum purification was evident.

Keywords：Chicken dysenterydetection；Plasma residual fluid;Frozen conditions

雞白痢沙門氏菌（SalmonellaPullorum，S.Pullorum）是一種嚴(yán)重危害家禽健康的病原菌，對(duì)雞群危害較大，受感染的雛雞急性發(fā)病后死亡率較高，即使雞群耐受后，其生長(zhǎng)也受到影響，給養(yǎng)殖業(yè)帶來嚴(yán)重經(jīng)濟(jì)損失[1]。（剩余8005字）