羅伊氏菌素對(duì)豬腸上皮細(xì)胞免疫調(diào)控研究及其在仔豬健康中的潛在應(yīng)用

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中圖分類號(hào)：S816.73 文獻(xiàn)標(biāo)識(shí)碼：A 文章編碼：1005-8567（2025）03-0053-10

Abstract：The weaning stage of piglets is confronted with challenges such as imperfect intestinal development， unbalanced microbial community，andan immature immune system，resulting in frequent intestinal diseasesand severelyafecting growthperformance.Thesearch foralternatives toantibioticsisurgent.Inthisstudy，Lactobacills reuteriLR1strainwasisolatedfromthefecesofhealthyweanedpiglets，anditsbiologicalcharacteristicswere analyzed，andtheefectofits metabolitereuterinonporcineintestinal epithelialcells（IPEC-J2）was explored.The results showed thattheLR1 strain grew bestat39.25Cand pH=6.387；reuterinhadsignificant antibacterial activity againstETEC K88，and the inhibitory efect increased with the increase of concentration；it hada time-and concentration-dependent effectonthesurvivalrateof IPEC-J2cell，withcytotoxicityintheearly stageandan increase in survival ratein the laterstage；different concentrationsof reuterin（2 ，20 μL/mL ， 50μL/mL ）

inducedthedifferentialexpressionof interleukinsand chemokines in IPEC-J2cels.This studyprovidesatheoretical basis fortheapplicationofreuterinasanantibiotic-free feedaditiveinpigletbreedingand helps topromotethegreen and healthy development of the livestock industry.

Keywords：Reuterin；Porcine intestinal epithelial cells；Immune regulation；Probiotic metabolite

在現(xiàn)代畜牧業(yè)中，仔豬斷奶階段至關(guān)重要，同時(shí)也面臨諸多挑戰(zhàn)。（剩余12134字）