延遲激活及不同處理方式對豬體細胞克隆胚胎體外、體內(nèi)發(fā)育效率的影響

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中圖分類號： 5821.3+6 文獻標識碼：A文章編碼：1005-8567（2025）03-0033-06

Abstract：The literature shows that electrofusion parameters，fusion methods and treatment methods of cloned embryosmayafecttheinvitrodevelopmentefficiencyofporcinecloned embryos.Theeffctsofsynchronousactivation anddelayedactivationoninvitroandinvivodevelopment eficiencyof porcinecell cloned embryos werecompared. All embryos were placed in PZM-3 culture medium containing 5μg/mL of cytochalasin B（CB） for 4h after activation. There was no significant difference in the fusion rate between the two groups（ P>0.05 ），but the cleavage rate and blastocyst development rate of delayed activation were higher than those of synchronous activation group.CB and histonedeacetylase inhibitor arealsocommonlyused inthe treatment ofactivated cloned embryos.This study also comparedthe treatmentwithcytorelaxinBafterdelayedactivationandthetreatmentwith histonedeacetylaseinhibitor scriptaid，andfoundthatthelatter was moreconduciveto improving theefficiencyofinvitro embryodevelopment.The cloned embryosobtainedbytheabovedifferent methods were transferred，andthe statisticalresultsshowedthat the delayed activation method was moreconducive to embryodevelopment.After delayedactivation，CBor scriptaid was usedtotreat theembryos，andthepregnancyrateand cloning eficiencyaftertransplantation were significantlyhigher than those in the synchronous activation group.

Keywords：Pig；Somatic cellcloning；Delayed activation；Processing mode；Developmental efficien

豬體細胞克隆在動物育種、挽救瀕危動物和動物醫(yī)學模型上都有廣闊的發(fā)展前景，但目前成功率依然很低。（剩余9624字）