pll</sub> 基因?qū)儆谥楸话l(fā)育異常蛋白(ANT)轉(zhuǎn)錄因子基因，對植株生長、果實(shí)發(fā)育等具有調(diào)控作用。本研究使用病毒誘導(dǎo)的基因沉默(VIGS)技術(shù)對普通葉型甜瓜植株的 <sub>pll</sub> 基因進(jìn)行基因沉默處理，對比在pll基因正常表達(dá)與沉默表達(dá)的情況下，甜瓜果實(shí)中脂氧合酶活性、乙烯釋放與呼吸代謝的變化，并研究 <sub>pll</sub> 基因表達(dá)受到沉默抑制時(shí)，對果實(shí)中乙烯合成與糖代謝相關(guān)酶編碼基因表達(dá)及相關(guān)酶活性產(chǎn)生的影響。結(jié)果表明，當(dāng)果實(shí)中 <sub>pll</sub> 基因的表達(dá)水平較低時(shí)，能夠降低脂氧合酶活性、乙烯釋放量與呼吸速率，并且 <sub>pll</sub> 基因?qū)麑?shí)中乙烯合成與糖代謝相關(guān)酶編碼基因的表達(dá)有正向調(diào)控作用，當(dāng) <sub>pll</sub> 基因的表達(dá)受到沉默抑制時(shí)，果實(shí)中乙烯合成、糖代謝相關(guān)基因的相對表達(dá)量亦隨之下調(diào)，從而降低乙烯合成與糖代謝相關(guān)酶的活性。由此可知， <sub>pll</sub> 基因可能參與果實(shí)后熟生理的調(diào)控，并且 <sub>pll</sub> 相對表達(dá)量的下調(diào)對果實(shí)后熟生理代謝有延遲、抑制作用。-龍?jiān)雌诳W(wǎng)" />

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甜瓜掌狀裂葉p基因?qū)A藏期果實(shí)后熟生理的調(diào)控作用

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Regulatory effect of the palmate-lobed leaf gene pll on the post-harvest physiology of melon fruits during storage

ZHANG Qiang1， YONG Liangmin1， DAI Wenting2， SHI Yunjiao1

（1.Hinanlsfse

al Sciences，Haikou 571100，China）

Abstract： The melon palmate-lobed leaf gene pll belongs to AINTEGUMENTA（ANT） transcription factor gene， which playsan importantrole inregulating plant growthand fruit development.During theresearch，the gene silencing of pll was detected byvirus-induced gene silencing（VIGS）technique.Thechanges of lipoxygenase activity，ethylene release and respiratory metabolism in fruits under normal and silent expression of pll gene were compared，and the effects of pl （204號 gene silencing inhibitiononthe expression of ethylene synthesisand sugar metabolism enzyme-encoding genes and related enzyme activities in fruitswere studied.The results showed that the low expression of pll gene in fruits could reduce the activityof lipoxygenase，ethylene release and respiration rate.Moreover，the pll gene had a positive regulation efect on ethylene synthesis and carbohydrate metabolism gene expression in fruits，and when the expression of pll gene was suppressed by silencing，the expresion of genes related to ethylene synthesisand glucose metabolism in fruits was also dow-regulated， thus reducing the activities of ethylene synthesis and glucose metabolism enzymes.It can be seen that the pll gene is in

收稿日期：2024-10-29

volvedintheregulationofpost-harvestphysiology，andthe down-regulation of pll gene expression has a delayed and inhibitory effect on the post-harvest physiological metabolismof melon fruits.

Key words：muskmelon； pll gene； gene silen-cing；enzymeactivity；post-harvest physiology

甜瓜（CucumismeloL.）主要產(chǎn)于中國西部地區(qū)，其農(nóng)藝性狀優(yōu)良，果型勻稱，口感與風(fēng)味俱佳，深受消費(fèi)者喜愛。（剩余11405字）

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