支鏈氨基酸轉(zhuǎn)氨酶1在口腔鱗狀細(xì)胞癌中的表達(dá)及其功能研究

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[中圖分類號(hào)]R739.8[文獻(xiàn)標(biāo)志碼]A[doi]10.7518/gjkq.2025045

Expressionand function of branch-chain amino acid transaminase1 inoral squamous cellcarcinoma

LiYijie'，YuanZhenying'，LiMing2

1.Dept.ofentaltheticsgshatomtologicalHospitalangsh，ina；2.gaolal

ofStomatologyCentralSouthUnversityKeyLaboratoryofOralHealthesearchinHunanProvinceangsh410000，China

Supported by：Key Research and Development Project of Hunan Province （2022SK2052）

Correspondence：LiMing，Email： limingq248@163.com

[Abstract]ObjectiveThis study aimed to analyze the expressonand biological functionofbranch-chain amino acid transaminase1（BCAT1） inoral squamous cellcarcinoma （OSCC）.MethodsThe expression of BCAT1 in head and neck squamous cellcarcinoma （HNSC）patients and its relationshipwith prognosis were analyzed by bioinformatics.To detect BCAT1 gene expresson in tumor and adjacent tisues，we selected patients with OSCC diagnosed in the Departmentof Oraland Maxillfacial Surgery，XiangyaStomatology Hospital，Central South University from2021to2022. BCATl gene expresson in human OSCCceline and normal oral keratinocytes was analyzed.After plasmid overexpressionor knockdownofBCAT1 gene，the proliferation，migration，and invasionabilityof human OSCCcels were detected by cellproliferation，wound-healing，and invasionassys，respectively.ResultsBCAT1 was highly expressed incancer tisues of HNSC patients in the Cancer Genome Atlas （TCGA）databaseand was one of the independent prognostic factors and potential diagnostic markers of patients.Results ofcellularassays showed thatBCAT1 expression wasupregulated in human OSCCtissues orcelines，therebyregulating theproliferation，migration，and invasionofOSCCcels.Con

clusion BCAT1 gene is a potential therapeutic target for human OSCC.

[Keywords] branch-chain amino acid transaminase 1； oral squamous cell carcinoma； head and neck squamous cell carcinoma； proliferation； migration； invasion

口腔鱗狀細(xì)胞癌（oralsquamouscell carcino-ma，OSCC）是頭頸部最常見的癌癥類型之一。（剩余14491字）