50</sub>=(0.108±0.002)mg/mL) 的清除能力最強(qiáng)，丙酮提取物對(duì)ABTS自由基 (IC<sub>50</sub>=(0.094±0.001)mg/mL) 的清除能力最強(qiáng)，乙酸乙酯提取物對(duì)羥自由基 (IC<sub>50</sub>=(0.105±0.005)mg/mL) 的清除能力最強(qiáng)；清除亞硝酸鹽結(jié)果顯示，甲醇提取物對(duì)亞硝酸鹽清除率最強(qiáng)，為 57.86% 。在抑菌方面，不同溶劑提取物對(duì)兩種實(shí)驗(yàn)菌表現(xiàn)出不同的抑菌效果，其中無(wú)水乙醇提取物的抑菌效果最佳，且花椒葉不同溶劑提取物對(duì)金黃色葡萄球菌的抑制效果相對(duì)較好。研究發(fā)現(xiàn)，花椒葉提取物具有一定的抗氧化活性、清除亞硝酸鹽能力、抑菌活性，也進(jìn)一步為花椒葉的開發(fā)、提取、利用奠定了一定的理論依據(jù)。-龍?jiān)雌诳W(wǎng)" />

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花椒葉不同溶劑提取物抗氧化、清除亞硝酸鹽及抑菌活性對(duì)比研究

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DOI：10.3969/j.issn.1000-9973.2025.07.012

Comparative Study on Antioxidant，Nitrite Scavenging and Antibacterial Activities of Different Solvent Extracts from

Zanthoxylum bungeanum Leaves CHEN Xin-yu1，ZHANG Xue1，XIE Zhen-jian1，2* （1.College of Food and Biological Engineering，Chengdu University， Chengdu 61OlO6，China; 2.Department of Science and Technology（Transformation Center of Scientific and Technological Achievements），Chengdu University，Chengdu 6lo1o6，China）

Abstract： In this study，with Zanthoxylum bungeanum leaves as the raw materials，ultrasonic extraction is performed on Zanthoxylum bungeanum leaves using different solvents （water， methanol，anhydrous ethanol，acetone，n-butanol，ethyl acetate），the content of active components in different solvent extracts are determined，and the efects of antioxidant activity，nitrite scavenging abilityand antibacterial activitiy are investigated. The results show that the content of total polyphenols in methanol extract is the highest of （94.77±0.56）mg/g ，and the content of total flavonoids in acetone extract is the highest of （32.47±0.24）mg/g The antioxidant activity results show that methanol extract has the strongest scavenging ability against DPPH radicals （IC50=（0.108±0.002）mg/mL） ，acetone extract has the strongest scavenging ability against ABTS radicals （IC50=（0.094±0.001）mg/mL） ， and ethyl acetate extract has the strongest scavenging ability against hydroxyl radicals （IC50=（0.105±0.005）mg/mL） . The nitrite scavenging results show that the methanol extract has the strongest nitrite scavenging rate，which is 57.86% . In terms of bacteriostasis，different solvent extracts show diffrent bacteriostatic efects on the two experimental bacteria，among which，anhydrous ethanol extract has the best bacteriostatic effct，and different solvent extracts of Zanthoxylum bungeanum leaves have relatively good inhibitory efect on Staphylococcus aureus.Through the study，it is found that the extracts of Zanthoxylum bungeanum leaves have certain antioxidant activity， nitrite scavenging ability and bacteriostatic activity，which has further laid a certain theoretical basis for the development，extraction and utilization of Zanthoxylum bungeanum leaves.

Key Words： Zanthoxylum bungeanum leaves；total polyphenols；total flavonoids；antioxidantactivity； nitrite; antibacterial activity

花椒（ZanthoxylumbungeanumMaxim.）屬于蕓香科、花椒屬落葉小喬木植物，花椒被譽(yù)為“調(diào)味之王”，具有固麻、增香、去腥、去膻等功效，還具有一定藥用價(jià)值，主要分布于中國(guó)四川、貴州、云南等地區(qū)[2]。（剩余14301字）

目錄
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