18</sub> (2(MGII) ( 4.6mm×250mm,5μm )色譜柱,以甲醇-乙腈 -0.2% 磷酸溶液為流動(dòng)相,梯度洗脫;柱溫30℃;檢測(cè)波長(zhǎng)為280、210、230、330、403nm ,流速為 1.0mL/min ,進(jìn)樣量 20μL ;建立15批桃紅四物湯物質(zhì)基準(zhǔn)特征圖譜,并同步測(cè)定12種成分含量。[結(jié)果]15批桃紅四物湯物質(zhì)基準(zhǔn)特征圖譜相似度大于0.9,確定16個(gè)共有峰,指認(rèn)出13個(gè)色譜峰,12種成分在一定濃度范圍內(nèi)線性關(guān)系良好( R<sup>2</sup>? 0.999 8)。[結(jié)論]建立的經(jīng)典名方桃紅四物湯特征譜圖和12種成分含量測(cè)定方法準(zhǔn)確可靠,為經(jīng)典名方質(zhì)量控制研究提供參考。-龍?jiān)雌诳W(wǎng)" />

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桃紅四物湯特征圖譜及多成分含量研究

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關(guān)鍵詞經(jīng)典名方;桃紅四物湯;特征圖譜;多中圖分類號(hào)R286 文獻(xiàn)標(biāo)識(shí)碼A文章編號(hào) 0517-6611(2025)13-0170-04d . issn. 0517-6611.2025.13. 032開(kāi)放科學(xué)(資源服務(wù))標(biāo)識(shí)碼(OSID):

Study on the Characteristic Chromatogram and Determination of Multi-component Content of Taohong Siwu Decoction ZHU Qi-liang,ZHOU Quan2,CAOLan-lan2etal(1.GuangzhouBaiyunshanzhongyiPharmaceuticalCo.,Ld.,Guangzhou,Guangdong 510530;2.Shanghai R&D Center for Standardization of Chinese Medicines,Shanghai )

AbstractObectiveTostablishamethodforcharacteristicromatoamanddetermiatioofulticompoentsofTaohongSiDeco tion.[Method]CAPCELL PAK C18 (MGII) column( 250nm×4.6nm,5μm) )was used and the mobile-phase was methanol-acetonitrile -0.2% phosphoric acid solution with gradient elution. The column temperature was 30qC . The detection wavelengths were 280,210,230,330 and 403 nm. The flow rate was 1.0mL/min and the injection volume was 20μL . The similarity evaluation combined with cluster analysis was used toevaluateteharacteristicapof5batsofaohongSiuDecoctioandteotentsof1omponntsredeteindiulta ously.Resultesilarityfebcharkcharacteristcspectraof15batchsofTohongSiuDecoctionasgeaterthanO.9and16 commonpeakswerealibratedandatotalof13hromatographicpeakswereidentifed.The1omponentssowedgodlinearelatiosis within a certain concentration range ( ).[Conclusion]The established method for characteristic chromatogram and content determinationofmultcomponntsofTaohongSiuDecoctionereacurateandreliable,hichanprovidareferenceforfurtqualityotol valuation of traditional classical formula.

KeywordTraditioalclasicfoula;TaongSiDecotion;Characteritichoatoga;Multi-opoents;Contentdeteatio

桃紅四物湯出自清朝柴得華所撰婦科名著《婦科冰鑒》,該方由當(dāng)歸、地黃、川芎、白芍、桃仁、紅花6味藥組成[1]。(剩余5224字)

目錄
monitor